What is mCherry?
mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with low acid sensitivity. compare Comparison List Add mCherry show comparison clear selection FPbase info
What is the mass of the mCherry protein?
The gene for mCherry is 711bp long, and the protein is made up of 236 residues with a mass of 26.722 kDa. The crystal structure of mCherry was determined in 2006.
What does 135 mCherry mean?
Add to favorites135 mCherry is a basic (constitutively fluorescent) red fluorescent protein published in 2004, derived from Discosoma sp.. It is reported to be a very rapidly-maturing monomer with low acid sensitivity. Oligomerization Organism Molecular Weight Cofactor Monomer Discosoma sp. 26.7 kDa FPbase ID: ZERB6 Attributes Ex λ Em λ
What is the crystal structure of mCherry?
The crystal structure of mCherry was determined in 2006. It contains 3 alpha helices and 13 beta sheets which make up the beta barrel. The chromophore in mCherry is made up of three amino acids, methionine, tyrosine, and glycine, which are post-translationally modified into an imidazolinone.
What size is mCherry?
mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).
What is the overall structure of mCherry?
The structure of mCherry protein consists of 3 alpha helices and the beta barrel that is made of 13 beta sheets. Its chromophore contains three amino acids, glycine, methionine and tyrosine. These amino acids are post-translationally modified into imidazoline.
How big is a GFP?
GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence and therefore GFP cannot be downsized by deleting residues.
How big is GFP kDa?
The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa.
What is the difference between GFP and mCherry?
As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequoera victoria jellyfish. Fluorescent proteins are used to tag components in the cell, so they can be studied using fluorescence spectroscopy and fluorescence microscopy.
Is mCherry and RFP same?
mCherry is brighter than RFP, but the literature suggests that mRuby is one of the brightest versions of RFP.
What is the smallest fluorescent protein?
miRFP670nanoWith molecular weight of only 17 kDa, miRFP670nano is the smallest monomeric NIR FP that fluoresces in mammalian cells as bright as twice bigger state-of-art two-domain NIR FPs.
Does mCherry need oxygen?
One of the major differences in the maturation process is the need for two moles of molecular oxygen to form a mature mCherry chromophore, compared to the one mole required for GFP [3, 14].
What microscope can see GFP?
Flow cytometry and fluorescent microscopy are two conventional tools to detect the GFP signal; flow cytometry is an effective and sensitive technique to quantitatively analyze fluorescent intensity, while fluorescent microscopy can visualize the subcellular location and expression of GFP.
What is the molecular weight of mCherry?
28 kDamCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel (3).
How many kDa is GST GFP?
about 54 KDaThe GST-superfolder GFP fusion protein is about 54 KDa in size.
How large is RFP in kDa?
27 kDaRed Fluorescent Protein (RFP) Protein Protein The molecular weight of RFP (calculated) is 27 kDa, but in a gel it is expected to run at approximately 28-30 kDa.
What is the mCherry gene?
mCherry is a red fluorescent protein (RFP), classified as a luminescent protein, that belongs to a group of fluorescent protein chromophores. mCherry is a part of the mFruits protein family, which is a family of mRFPs, monomeric red fluorescent proteins.
Is mCherry a monomer?
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon after transfection or activation of transcription. It is highly photostable and resistant to photobleaching (Shaner et al. 2004).
Where is mCherry from?
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in transfection and transgenic experiments.
How do you detect mCherry?
It'll depend on the flow cytometer you have access to but to best detect the mCherry signal excite with the yellow-green laser at 561 nm and detect in the PE-TexasRed channel with a 610/20 bandpass filter.
How long is the mcherry gene?from en.wikipedia.org
The gene for mCherry is 711bp long, and the protein is made up of 236 residues with a mass of 26.722 kDa. The crystal structure of mCherry was determined in 2006. It contains 3 alpha helices and 13 beta sheets which make up the beta barrel. The chromophore in mCherry is made up of three amino acids, methionine, tyrosine, and glycine, which are post-translationally modified into an imidazolinone. The number of these residues in sequence are 71, 72, and 73 respectively. The extended pi-electron conjugation gives mCherry its red-shifted absorbance and emission. The chromophore forms from a central helix which is shielded from solvent in an 11-stranded beta barrel. This structure is almost identical to the tertiary structure of DsRed which also has an 11-stranded beta barrel, and is similar to GFPs tertiary structure. This makes the environment around the chromophore in mCherry more hydrophobic than the environment around the chromophore of DsRed. The end termini on mCherry are GFP-like which allows it to be incorporated in to systems where GFP can be used and mRFP1 could not have been used.
What is the wavelength of mcherry?from takarabio.com
PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm.
What is mcherry protein?from takarabio.com
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon after transfection or activation of transcription. It is highly photostable and resistant to photobleaching (Shaner et al. 2004). As a result, mCherry is now the most widely ...
Why is mcherry important?from en.wikipedia.org
mCherry is valued where constitutive gene expression is desired, and other experimental approaches require the coordinated control of multiple genes. While multiple venues have been developed for use in E. coli and other models, the utility and functionality of such techniques does not always translate to other species.
What are the amino acids in mcherry?from en.wikipedia.org
The chromophore in mCherry is made up of three amino acids, methionine, tyrosine, and glycine, which are post-translationally modified into an imidazolinone. The number of these residues in sequence are 71, 72, and 73 respectively. The extended pi-electron conjugation gives mCherry its red-shifted absorbance and emission.
What is the pEF1-mCherry-N1 vector?from takarabio.com
pEF1α-mCherry-N1 is a mammalian expression vector that constitutively expresses a protein of interest fused to the Nterminus of the red fluorescent protein mCherry, even after stable integration of the vector into the host cell genome.
What is a lentiviral particle?from takarabio.com
Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAm Cherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm.
How tall can a black cherry tree grow?
In North America, you can find a wild cherry tree known as the black cherry that can grow up to 80 feet tall. The black cherry tree can also grow to be 60 feet wide, and it’s definitely a sight to behold.
How tall do sour cherry trees get?
You can also find semi-dwarf and dwarf variants of these trees. Semi-dwarf sour cherry trees can grow up to 12 feet tall whereas dwarf sour cherry trees will grow up to 8 feet tall. If you don’t want a cherry tree that is quite so big, then going with a sour cherry tree might be your best bet.
Why are cherry trees so popular?
Cherry trees are popular for a number of different reasons. You might be interested in planting cherry trees in your yard simply because of how gorgeous they look. Other people might want to plant cherry trees because they’re interested in being able to harvest cherries each season. Either way, it’s easy to see why cherry trees are so appealing ...
What is the best cherry tree for a 5-7 climate?
The Higan cherry is going to thrive in USDA zones 5-8 while the Amanogawa thrives in USDA zones 5-7. These two ornamental cherry trees are very practical for many people and you should consider them if you live in the right climate. Another popular type of ornamental cherry tree is the Yoshino cherry tree.
What zone do cherry trees grow in?
Wild cherry trees such as this thrive in USDA zones 2-8. If you have one of these trees on your property, you should keep in mind that the bark, roots, seeds, stems, and leaves are poisonous.
What is the difference between a rainier cherry tree and a sweet cherry tree?
There are other variants to consider as well that are a bit different than the standard sweet cherry trees. Rainier cherry trees grow sweet yellow cherries, and they grow to comparable sizes as other sweet cherry trees. The big difference with the rainier cherry trees will be the fruit color and that they can thrive in USDA zones 5-9.
What kind of soil do cherry trees need?
One of the first things to consider is the type of soil that you’re planting the trees in. Cherry trees are going to need well-draining soil, and soil with high clay content simply won’t do. You might need to prepare the soil for the tree ahead of time if you want the cherry tree to do well.
What is the wavelength of mcherry?
PAmCherry is non-fluorescence until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm. The excitation/emission wavelengths of photoactivated PAmCherry are 564 nm and 595 nm.
What is mcherry protein?
mCherry is a bright red monomeric fluorescent protein created by rounds of directed evolution of DsRed. mCherry matures rapidly, making it possible to see results very soon after transfection or activation of transcription. It is highly photostable and resistant to photobleaching (Shaner et al. 2004). As a result, mCherry is now the most widely ...
What is the pEF1-mCherry-N1 vector?
pEF1α-mCherry-N1 is a mammalian expression vector that constitutively expresses a protein of interest fused to the Nterminus of the red fluorescent protein mCherry, even after stable integration of the vector into the host cell genome.
What is the expression vector of mcherry?
pEF1α-mCherry-C1 is a mammalian expression vector that constitutively expresses a protein of interest fus ed to the C-terminus of the red fluorescent protein mCherry, even after stable integration of the vector into the host cell genome.
What is a lentiviral particle?
Lentiviral particles derived from the vector allow you to infect cells and express your gene of interest fused to PAm Cherry, a photoactivatable mutant of the fluorescent protein mCherry. PAmCherry is non-fluorescent until photoactivated by a short exposure to light at a wavelength between 350 nm and 400 nm.
Where does mcherry come from?
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish , sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
What is mcherry protein?
mCherry is the second generation monomeric red fluorescent protein that has improved brightness and photostability. The protein is suitable as a positive control for mCherry protein expression studies or as a labeling reagent.
What is the row size of cherry?
You may receive cherries marked as “12 Row”, “11 Row” or even in half rows, such as “10-1/2 Row” or “11-1/2 Row’. These row sizes do have specific size designations. You may find it useful to size the cherries using one of the cherry sizers. The first sizer pictured below is the cherry sizer used for cherries packed and shipped from California.
How to measure cherries?
The cherries are measured by holding the cherry by the stem and dropping it through the designated hole. If the cherry passes through the hole, the cherry is considered undersize. If you have a problem with the size you would want to count out 50 cherries at random and measure each one. If 3 cherries passed through the sizer, based on 50 count, you would have 6% of the cherries being undersized, exceeding the tolerance for undersize.
What size are cherry boxes?
You may receive cherries marked as “12 Row”, “11 Row” or even in half rows, such as “10-1/2 Row” or “11-1/2 Row’. These row sizes do have specific size designations.
Where are cherry sizers shipped from?
The first sizer pictured below is the cherry sizer used for cherries packed and shipped from California. The second sizer, pictured below is the sizer used for cherries originating from Washington State.
What is the size of a California sizer?
The California Sizer has a few more row size designations, (12-1/2, 13 and 14 Row), but the minimum sizes for the remaining sizes are the same. For example; if you receive sweet cherries marked 12 Row, that means not more than 5% of the cherries may be smaller than 54/64 of an inch.
