Aseptic techniques
- Close windows and doors to reduce draughts and prevent sudden movements which might disturb the air.
- Make transfers over a disinfected surface. ...
- Start the operations only when all apparatus and materials are within immediate reach.
- Complete all operations as quickly as possible, but without any hurry.
- Vessels must be open for the minimum amount of time possible.
- Close windows and doors to reduce draughts and prevent sudden movements which might disturb the air.
- Make transfers over a disinfected surface. ...
- Start the operations only when all apparatus and materials are within immediate reach.
- Complete all operations as quickly as possible, but without any hurry.
What are aseptic techniques?
Aseptic techniques are methods that prevent the growth of unwanted microorganisms. This is important in practice as it prevents contamination and the growth of pathogenic microorganisms. Aseptic techniques include disinfecting work surfaces, using a bunsen burner and flaming bottleneck of bacterial samples.
What are the precautions to be taken when performing aseptic procedures?
effectively sterilizing inoculating loops and other equipment that comes into contact with cultures or media, and avoiding breathing on cultures or sterile instruments. There are some general rules to follow for any aseptic technique. Close windows and doors to reduce draughts and prevent sudden movements which might disturb the air.
How do you manage an aseptic field?
the main aseptic field does not have to be managed as a key part non sterile gloves can be used unless key parts must be touched, when sterile gloves must be worn. Perform hand hygiene. Clean trolley/work surface with detergent and water or detergent wipe. Identify and gather equipment for procedure. drop sterile equipment into sterile field.
What are the four aseptic techniques used in catheterization?
When healthcare providers insert a catheter, they demonstrate all four aseptic techniques in action: Barriers: They wear sterile gloves. Patient and equipment preparation: They open sterile packaging that contains the sterile catheter. They prepare the patient’s skin with a special solution.
How do you do an aseptic technique?
Aseptic preparation may involve: disinfecting a patient's skin using antiseptic wipes. sterilizing equipment and instruments before a procedure. keeping sterilized instruments inside plastic wrappers to prevent contamination before use.
What are the 5 aseptic techniques?
Aseptic technique types. According to The Joint Commission, there are four chief aspects of the aseptic technique: barriers, patient equipment and preparation, environmental controls, and contact guidelines. Each plays an important role in infection prevention during a medical procedure.
How do you perform aseptic techniques in microbiology?
Specific Aseptic TechniquesAlways wipe your hands and work area with 70% ethanol.It is recommended to wear gloves. ... Wipe the outside of the containers, flasks, plates, and dishes with 70% ethanol before placing them in the cell culture hood.Avoid pouring media and reagents directly from bottles or flasks.More items...•
How does carrying out aseptic technique ensure safety?
Infection prevention and control In turn, aseptic technique minimises the risk of contamination of key sites, protects patients from their own pathogenic microorganisms, and ultimately, reduces the risk of infection transmission.
What are the 8 principles of sterile technique?
Terms in this set (8)Don't Touch. Only sterile can touch sterile and remain sterile.Don't Add. Only sterile objects can be put on a sterile field to remain a sterile field.Keep in Sight. Keep the sterile field in sight.Keep High. Keep field at or above waist level at all times.Time. ... No Water. ... 1 Inch Border. ... Don't Intrude.
What do you mean by aseptic?
aseptic • \ay-SEP-tik\ • adjective. 1 a : preventing infection b : free or freed from pathogenic microorganisms 2 : lacking vitality, emotion, or warmth. Examples: Patients with compromised immune systems must be treated in aseptic environments. "[
Why is aseptic technique used in the laboratory?
In the microbiology lab we use aseptic technique to: Prevent contamination of the specific microorganism we are working with. Prevent contamination of the room and personnel with the microorganism we are working with.
Why is aseptic technique important in the laboratory?
Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. Proper aseptic technique prevents contamination of cultures from foreign bacteria inherent in the environment.
Which procedures are performed using aseptic technique quizlet?
Aseptic Technique is a method provided to prevent any contamination from anything on the surface. What can Aseptic Technique be good for ? Anything to do with Surgery, Catheters, Aterial Lines, Draining, Surgical Technique.
What is aseptic technique and when is it used?
An aseptic technique is used to carry out a procedure in a way that minimises the risk of contaminating an invasive device, e.g. urinary catheter, or a susceptible body site such as the bladder or a wound.
When should you use aseptic technique?
An aseptic technique ensures that susceptible body sites (such as an open wound or insertion site for an invasive medical device) and the sterile parts of devices in contact with susceptible sites, are not contaminated during procedures such as wound dressings, urethral catheterisation or insertion of an intravenous ...
What are key parts in aseptic technique?
Key Parts - Key parts are the sterile components of equipment used during a procedure. Examples: bungs, needle hubs, syringe tips, dressing packs etc. Key Sites - Key sites include any non-intact skin and insertion or access sites for medical devices connected to the patient.
What are the 3 main risks of contamination during aseptic technique?
MatchThrough the air environment - airborne contamination.Via the healthcare worker - hand touch contamination.Via the procedure working space - direct and indirect contact contamination.
What are some aseptic techniques used to prevent contamination?
Other examplesNo eating or drinking in the lab.Wiping surfaces with disinfectant/alcohol.Not growing microorganisms at body temperature.Using sterile loops when transferring cultures.Flaming culture bottle necks to prevent contamination.Sterilizing (using an autoclave) or disposing of all used equipment.More items...•
What is the difference between sterile and aseptic technique?
Although aseptic and sterile both basically mean “germ-free,” sterile is more likely to describe medical environments, products, and instruments that have been cleaned (sterilized). Aseptic is more likely to describe techniques that keep an environment in its sterile state.
What is aseptic technique NHS?
Aseptic technique An aseptic technique ensures that only uncontaminated equipment and fluids come into contact with susceptible body sites. It should be used during any clinical procedure that bypasses the body's natural defences.
What is the importance of aseptic techniques?
Aseptic techniques ensure that bacterial samples are not contaminated. They also ensure that pathogenic microorganisms do not grow.
Identify 4 examples of aseptic techniques.
Wash hands before handling agar plates. Disinfect workspaces with an antibacterial cleanser. Use a bunsen burner to flame wire hoops. Flame bottle...
Why are bunsen burners used in aseptic techniques?
Bunsen burners prevent the entry of microorganisms into your culture.
What piece of equipment is used to transfer bacteria into agar plates?
Flamed wire hoops.
Identify the 4 stages of microbial growth. Under what conditions does the microbial growth curve occur?
The lag phase, exponential (log) phase, stationary phase and death phase. This occurs in a closed system.
Describe the lag phase of microbial growth.
The lag phase involves a small number of bacteria adjusting to their environment. There is very little growth happening at this stage.
Describe the exponential phase of microbial growth.
The exponential phase involves the rapid increase of the bacterial population due to binary fission. The bacteria grow in optimal conditions.
Describe the exponential phase of microbial growth.
The exponential phase involves the rapid increase of the bacterial population due to binary fission. The bacteria grow in optimal conditions.
Describe the stationary phase of microbial growth.
The stationary phase involves the plateau of bacterial growth. At this stage, the rate of bacterial growth is equivalent to the rate of bacterial d...
What is the purpose of aseptic technique?
Aseptic technique is an important infection prevention competency for protecting patients from healthcare-associated infection 8).
What is a micro critical aseptic field?
The micro critical aseptic field is a smaller version of the critical aseptic field and is used to protect a single key part. A micro critical aseptic field is usually the sterile packaging, cover, cap or sheath of a key part.
Why is critical aseptic field important?
A critical aseptic field ensures asepsis during procedures. It is used when key parts or key sites cannot be easily protected from contamination during a procedure. This may be because the key parts or key sites: can’t be protected by caps and covers. can’t be handled with non touch technique at all times.
What is the cause of preventable infections?
It is widely accepted that poor standards of aseptic technique are a major cause of preventable infections. Healthcare-associated infection is defined by The Association for Safe Aseptic Practice as an infection acquired by a patient as a consequence of exposure to pathogenic organisms via healthcare staff or healthcare facilities 9). In the European Union and European Economic Area it has been estimated that approximately 3.2 million patients per year will have at least one healthcare-associated infection 10). The Centers for Disease Control and Prevention (CDC) reports research indicating that healthcare providers and practitioners taking specific targeted steps can reduce some healthcare-associated infection (e.g. central-line associated bloodstream infections) by more than 70% 11).
What must an aseptic key part come into contact with?
Aseptic key parts must only come into contact with: other aseptic key parts. key sites. For example, the needle (key part) used to draw up a medication must only come into contact with a disinfected port on an IV line (an aseptic key part) or the intramuscular injection puncture site (a key site).
What are some examples of aseptic procedures?
Some examples of procedures requiring aseptic technique include: preparation and administration of intravenous (IV) fluids or medications. simple or complex wound dressings. insertion of urinary catheters. insertion of an intravenous (IV) cannula or central venous catheter (CVC) emptying or changing drainage bags.
What is an aseptic field?
An aseptic field is a designated work space that contains and protects procedural equipment from becoming contaminated. There are three types of aseptic fields. They are used in different situations and require different management. The choice of aseptic field should be made to ensure protection of key parts and sites.
How to sterilize a tube in an incinerator?
Hold the tubes closer to the bottom so that your hand will not be close to the flame when you sterilize the mouth of the tubes. Grab the inoculating loop far back on the handle as if you were going to write with it. Place your loop in the mouth of the incinerator briefly for 2-4 seconds to sterilize it.
How does a Bunsen gas burner work?
The knob underneath adjusts the amount of gas going into the burner tube. The barrel of the burner turns to adjust the amount of air going into the burner. Before lighting a Bunsen burner, set the air and gas adjustments to a minimal open position.
How to light a Bunsen burner?
Hold the cup part of the striker at an angle slightly above the opening of the burner and push the handle to generate a spark. You may need to repeat this several times before your burner lights. Alternatively, there may be butane lighters available in lab to light your Bunsen burner.
What is the aseptic technique?
Aseptic technique involves developing both manual dexterity in safely handling the microorganisms and mental dexterity in thinking ahead about what you are doing with the microorganism. In this lab you will learn how to: decontaminate your lab bench. safely organize your workspace. properly adjust your Bunsen burner.
Why is aseptic technique important?
Proper and appropriate aseptic technique is vitally important for the safety of all lab personnel; it is also essential for the successful completion of the lab portion of this class. The skills and awareness you develop practicing aseptic technique will carry over to your career as a health professional.
Why is it important to avoid aerosols?
One of your main concerns when working with microorganisms is to avoid producing aerosols that you can breathe in and droplets that can land on you, your lab partners, and your lab equipment. You will spend a lot of time in lab transferring organisms from one tube to another, or to slides or to plates . It is imperative that you do this quickly and safely. The longer your organism is exposed to the air, the more opportunities there are for it to get contaminated and/or to contaminate you, your lab partners or your equipment.
What temperature is the inside of a flame?
The tip of the little inside flame is the hottest part of the flame (1560°C). This is the area where you will want to run the top of your tubes through to maintain sterility.
What is the importance of aseptic techniques?
Aseptic techniques ensure that we do not contaminate our samples with microorganisms that we are not investigating. Our goal is to keep our microorganism samples pure. These techniques also discourage disease-causing microorganisms ( pathogens ), which are harmful to humans, from growing in a laboratory.
What are the aseptic techniques in microbiology?
Many aseptic techniques are used in a microbiology laboratory. Take a look at the list below.
Investigating the effect of antimicrobial substances on microbial growth
Antimicrobial substances describe chemical agents that kill bacteria. In this practical, we will investigate the use of these substances in culture and implement the aseptic techniques we learned earlier.
Aseptic Techniques
Aseptic techniques describe the methods by which a sterile environment is maintained to prevent the cultivation of unwanted microorganisms. The techniques include using an antibacterial disinfectant to wipe down laboratory surfaces, using a bunsen burner and using a flamed wire loop when transferring bacteria.
Why is my pipette leaking?
A leaking pipette is caused either by a faulty or ill-fitting teat, or by fibres from the cotton wool plug between the teat and the pipette. A dropping (Pasteur) pipette can be converted to deliver measured volumes by attaching it by rubber tubing to a non-sterile syringe barrel.
What is aseptic technique?
The aseptic techniques described here control the opportunities for contamination of cultures by microorganisms from the environment, or contamination of the environment by the microorganisms being handled. There are some general rules to follow for any aseptic technique.
Why is it important to use aseptic technique?
It is wise to treat all cultures as potentially pathogenic, because cultures may have been contaminated, and because mutations to disease-causing forms may occur.
What to do if pipette is contaminated?
f Immediately after use put the contaminated pipette into a nearby discard pot of disinfectant.
How to reduce chance of spores on plate?
d If you experience frequent contamination of plates with fungal spores, reduce the chance of draughts further, and consider inoculating plates from below with the agar surface facing downwards. In this way there is perhaps less chance of spores settling onto the plate from the air.
How to transfer fungus culture?
For transferring fungal cultures which grow by producing a mycelium of hyphae, an inoculation wire with the end bent into a small hook is better than a loop. Use the hook to gouge into the agar at the edge of the culture and pick up a small piece of agar plus hyphae. Transfer this to agar plate or slope, and invert the piece of fungus agar so that the fungus is in contact with the agar in the dish or tube. Ensure that the culture adheres firmly to the new agar. You may decide not to invert agar plates immediately in case the transferred culture falls off the agar.
What to do if a plug accidentally catches fire?
If a plug accidentally catches fire, douse the flames immediately by covering with a dry cloth, not by blowing or soaking in water.
What container do you use to transfer drugs in solution?
At a minimum, personnel should know how to transfer drugs in solution in vials or ampules into commonly utilized containers such as large volume parenterals (LVP) or small volume parenterals (SVP). Those techniques are highlighted in this section.
Why is sterile compounding important?
In sterile compounding processes, there is a substantial challenge to maintain microbial control of the environment. This is because personnel, even those who use good aseptic techniques, shed enormous numbers of particles from themselves and their clothing and these particles are laden with microorganisms. It is estimated that more than 99% of all microorganisms detected in clean rooms are of human origin. Therefore, special techniques, precautions, and tests are necessary to ensure the microbiological burden is reduced enough to cause no harm to the patient.
Why do nonaqueous drugs dissolve in water?
Generally, nonelectrolyte or neutral drugs (such as digoxin, phenytoin, and the benzodiazepines) are dissolved in a nonaqueous or a cosolvent vehicle due to their poor solubility in water. If the drug is placed in an aqueous environment, it may form a precipitate, with concomitant loss of drug activity and/or danger to the patient. The solvents used in nonaqueous parenteral products are usually listed on the product’s label. Hence, if the drug is dissolved in a water miscible solvent and one administers it slowly, dilution of the vehicle results in cosolvent fractions that maintain the drug in solution. If a cosolvent system must be used, the fraction of cosolvent in the initial stock solution should be as high as possible, to decrease the probability of precipitation upon administration.
How to position needle point on rubber closure?
Place the vial on the work area surface and position the needle point on the surface of the rubber closure so that the bevel is facing upward and the needle is at about a 45 – 60 degree angle to the closure surface .
What is the most effective method of preventing the transmission of pathogens that cause infection in the community and in health?
Hand hygiene , which is the most effective method of preventing the transmission of pathogens that cause infection in the community and in health-care settings, is an essential component of good compounding practices. Waterless, alcohol-based hand sanitizers that exhibit persistent activity (that is, effectiveness for six hours) and comply with guidelines and/or criteria from the USP, the FDA, and the Centers for Disease Control and Prevention (CDC), are among the most effective products that ensure an appropriate level of hand hygiene during compounding. In studies of antimicrobial-resistant organisms, alcohol-based products reduce the number of multidrug-resistant pathogens recovered from the hands of health-care workers more effectively than hand washing with soap and water.
Why do pharmaceuticals require special precautions?
Certain pharmaceutical preparations require special precautions in their preparation to minimize product contamination or environmental hazards. The following information may serve as a helpful guideline for a few of these classes of drugs.
What to wear when you have a swollen face?
Wear lint-free clothing or clothing covers, head and facial hair covers, and a mask.
How to avoid cross contamination?
Avoid pouring media and reagents directly from bottles or flasks. Use sterile glass or disposable plastic pipettes and a pipettor to work with liquids, and use each pipette only once to avoid cross contamination . Do not unwrap sterile pipettes until they are to be used. Keep pipettes at the work area.
What is aseptic technique?
Aseptic technique is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms (i.e., sepsis). While such actions are sometimes called “sterile technique,” that terminology is appropriate only in reference to preventing the introduction ...
What is a sterile graduated pipette?
Sterile graduated or dropping (Pasteur) pipettes are used to transfer cultures, sterile media and sterile solutions. Remove the pipette from its container/ wrapper by the end containing a cotton wool plug, taking care to touch as little of the pipette as you need to take a firm hold. Fit the teat.
Why is aseptic technique important?
Since the goal of a biologist is to grow microorganisms or eukaryotic cells without the introduction of extraneous organisms, aseptic techniques are crucial for accurate and meaningful experimentation. One should always remember that a completely sterile working environment does not exist.
Why seal multi-well plates?
Always cap the bottles and flasks after use and seal multi-well plates with tape or place them in resalable bags to prevent microorganisms and airborne contaminants from gaining entry.
What are some examples of aseptic techniques?
Examples of aseptic technique are: Cleaning and disinfecting lab surfaces prior to use. limiting the duration that cultures or media are uncapped ...
How to avoid breathing on cultures?
There are some general rules to follow for any aseptic technique. Close windows and doors to reduce draughts and prevent sudden movements which might disturb the air. Make transfers over a disinfected surface.
How to protect a wound from a desterilized dressing?
Wash your hands and put on sterile gloves. If the gloves become desterilised, remove them, re-wash your hands and put on new sterile gloves. This is best practice, but where resources are not available, safe modifications to this process can be made, for example by using non-sterile gloves to protect the nurse while removing the dressing and then washing the hands with gloves on and using alcohol gel on the gloves to make them clean enough to clean the wound and redo the dressing. This then protects both the nurse and the patient.
How to dispose of old dressing?
Wash your hands and put on non-sterile gloves (to protect yourself) before removing an old dressing. Dispose of this dressing in a separate dirty clinical waste bag.
Why do we use aseptic dressings?
Janet Marsden. When applying or changing dressings, an aseptic technique is used in order to avoid introducing infections into a wound. Even if a wound is already infected, an aseptic technique should be used as it is important that no further infection is introduced.
What is a document on a patient's chart?
Record (document) on the patient's chart your wound assessment, the dressing change and the care you have given.
What to do if gloves become desterilised?
If your gloves become desterilised, wash your hands and put on fresh gloves
How to introduce yourself to a patient?
Introduce yourself to the patient and explain what you are doing and why. If possible, provide privacy.
What is a wound assessment?
This includes a visual check and comparing and evaluating the smell, amount of blood or ooze (excretions) and their colour, and the size of the wound.
Introduction
Overview
- Aseptic technique involves developing both manual dexterity in safely handling the microorganisms and mental dexterity in thinking ahead about what you are doing with the microorganism. In this lab you will learn how to: 1. decontaminate your lab bench 2. safely organize your workspace 3. properly adjust your Bunsen burner 4. properly use an incine...
Part I - Procedures For Practice "Organisms"
- After you have practiced these procedures several times your instructor or IA will assess your proficiency. It is essential that you grasp these skills before you proceed to working with actual microorganisms.
Part II - Procedures For BSL2 Organisms
- Once your instructor or IA has observed your aseptic technique using the practice materials, you can begin to work with real organisms. The next step is learning proper aseptic technique for handling BSL 2 organisms.