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how do you get a sputum culture

by Dr. Petra Lind Sr. Published 3 years ago Updated 2 years ago
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A sputum culture requires minimal effort on your part. You simply need to provide the sample for the lab to test. You’ll be asked to cough deeply to bring up the sputum from your lungs. Saliva that can come up when someone is asked to cough is typically from the mouth and upper airways and isn’t useful for this test.

You will be asked to cough deeply and spit any phlegm that comes up from your lungs into a special container. The sample is sent to a lab. There, part of it is placed in a special dish (culture). It is then watched for two to three days or longer to see if bacteria or other disease-causing germs grow.

Full Answer

What can doctors learn from a sputum culture?

Sputum cultures can help a doctor diagnose pneumonia, bronchitis, and tuberculosis. A sputum culture can be used to test for fungus in the lungs. Doctors collect sputum by having the patient cough deep enough to produce phlegm, or sputum. The patient should then spit the sputum into a cup.

How to collect a sputum culture?

Taking a Sputum Culture Test

  • Before the test. The preparations for a sputum culture test can vary based on the kind of sample that is being collected.
  • During the test. The most common way of collecting a sputum sample is by coughing up phlegm and spitting it into a cup.
  • After the test. ...

What do you need to know about collecting sputum?

  • Instruct the patient to use the cotton ball or towelette to clean urethral area thoroughly to prevent external bacteria from entering the specimen.
  • Let the patient void into the container.
  • Label the specimen container with patient identifying information, and send to the lab immediately. ...
  • Wash your hands and instruct the patient to do it as well.

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What time of day is best to collect a sputum specimen?

Also Know, what is the best time of day to collect a sputum specimen? The best time to collect a specimen is in the morning, just after rising. If, however, you feel that you can produce a good specimen here at the hospital, we will be happy to assist you.

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How are sputum cultures obtained?

Coughing and spitting into a cup: The most common method of obtaining a sputum sample is by coughing deeply and then spitting the phlegm that comes up into a sample cup. This is generally the least invasive method of sputum culture testing.

How do you do a sputum culture test?

Your health care provider will need to get a sample of your sputum. During the test: A health care provider will ask you to breathe deeply and then cough deeply into a special cup. Your provider may tap you on the chest to help loosen sputum from your lungs.

When do you need a sputum culture?

But you might need to give a sputum culture if: Your cough suggests you have an illness caused by bacteria, such as bronchitis, pneumonia or tuberculosis (a potentially serious infection that usually affects your lungs and can cause you to cough up blood).

Why would a doctor order a sputum test?

A sputum test, also known as a sputum culture, is a test that your doctor may order when you have a respiratory tract infection or other lung-related disorder to determine what is growing in the lungs. Sputum is a thick substance that accumulates when bacteria or fungi grows and multiples in the lungs or bronchi.

How long does a sputum culture take for results?

Test results may take from 1 day to several weeks. How long your results take depends on the type of infection your doctor thinks you may have. Some organisms don't grow in a standard culture and need a special growth medium to be found in a sputum culture.

Is sputum test painful?

If you experience any of these symptoms, your doctor may recommend a sputum culture. This fast, relatively painless test helps laboratory technicians study the bacteria or fungi that might be growing in your lungs and causing the production of the sputum. This can help them find the cause of your illness.

What happens if sputum test is positive?

A sample of sputum is added to a substance that promotes the growth of bacteria. If no bacteria grow, the culture is negative. If bacteria grow, the culture is positive. If TB bacteria grow, then the person has tuberculosis.

Is sputum the same as phlegm?

Mucus in the lungs is known as phlegm or sputum. It is a common symptom in chronic lung diseases such as COPD (including chronic bronchitis and emphysema), cystic fibrosis, bronchiectasis, NTM lung disease or asthma.

What is the difference between mucus and sputum?

The term mucus may sometimes be used instead of sputum, but sputum refers to that mucus specifically secreted in the respiratory tract, whereas mucus may also be produced in the gastrointestinal tract, urological tract, and genital tract.

What is the best time of day to obtain a sputum specimen?

Sputum specimens should be collected in the early morning if possible. Collect 3 sputum specimens on 3 consecutive days unless otherwise instructed. Specimens should be kept in the refrigerator until they are submitted to the laboratory.

How much does a sputum culture cost?

How Much Does a Sputum Culture Cost? On MDsave, the cost of a Sputum Culture ranges from $15 to $72. Those on high deductible health plans or without insurance can save when they buy their procedure upfront through MDsave.

Are sputum cultures required to diagnose pneumonia?

Sputum culture is the most common test needed to be performed when the patient has pneumonia. It is used to identify the bacteria or fungi causing the airways or lung infection.

What is the best time of day to obtain a sputum specimen?

Sputum specimens should be collected in the early morning if possible. Collect 3 sputum specimens on 3 consecutive days unless otherwise instructed. Specimens should be kept in the refrigerator until they are submitted to the laboratory.

Which media is used for sputum culture?

Sputum culture – Selective and differential media: Since using all of these media adds to the cost of processing, many laboratories use a MacConkey medium or equivalent for preliminary differentiation of pseudomonas. Most patients with predominating P cepacia will be identified if semi-quantitative methods are used.

What is the difference between phlegm and sputum?

Sputum is not saliva but the thick mucus – sometimes called phlegm – which is coughed up from the lungs. The body produces mucus to keep the thin, delicate tissues of the respiratory tract moist so that small particles of foreign matter that may pose a threat can be trapped and forced out.

What is a sputum culture?

A sputum cultureis a sample of the gooey substance that often comes up from your chest when you have an infection in your lungsor airways. It is mostly made up of white blood cells that fight infection mixed with germs. Doctors use it to figure out what might be causing your illness, whether it’s bacteria, a virus or something else.

How much sputum do you need for a sputum test?

Your doctor will probably need about 1 teaspoon (5 milliliters) of sputum to run the test.

What causes a cough?

But you might need to give a sputum culture if: 1 Your cough suggests you have an illness caused by bacteria, such as bronchitis, pneumonia or tuberculosis (a potentially serious infection that usually affects your lungs and can cause you to cough up blood ). 2 Your cough might be caused by other microbes, such as a fungus or a virus.

What does it mean when your sputum is yellow?

It can give clues as to what’s going on: Off-white, yellow or green:This mean your sputum probably includes large numbers of infection-fighting white blood cells-- a sign of a respiratory illness such as pneumonia or bronchitis.

What is the body's sputum?

Why Your Body Makes Sputum. Your lungsare connected to your mouthby a passage called the trachea, or windpipe, which starts at the back of your throat. A few inches down, it splits into separate channels called bronchi, which funnel air from the trachea into your lungs.

Why do you need a test for infection?

Those tests will help separate the normal bacteria that are present in your body from the disease-causing bugs that may be making you sick. If an infection is found, additional tests can clarify what antibiotic to prescribe.

What is the instrument used to collect a sample?

In a more invasive procedure, they might have to use an instrument called a “bronchoscope” to collect a sample. The device has a light and a tiny camera. Your doctor gently inserts it down your windpipe to locate a sample.

What is a sputum culture?

A sputum culture is a test that checks for bacteria or another type of organism that may be causing an infection in your lungs or the airways leading to the lungs. Sputum, also known as phlegm, is a thick type of mucus made in your lungs. If you have an infection or chronic illness affecting the lungs or airways, it can make you cough up sputum.

What happens during a sputum culture?

Your health care provider will need to get a sample of your sputum. During the test:

Will I need to do anything to prepare for the test?

You may need to rinse your mouth out with water before the sample is taken. If you will be getting a bronchoscopy, you may be asked to fast ( not eat or drink) for one to two hours before the test.

What does it mean when sputum is clear?

Clear. This usually means no disease is present, but large amounts of clear sputum may be a sign of lung disease.

Why is sputum color important?

The thickness of sputum helps trap the foreign material. This allows cilia (tiny hairs) in the airways to push it through the mouth and be coughed out. Sputum can be one of several different colors. The colors can help identify the type of infection you may have or if a chronic illness has become worse: Clear.

What causes yellowish green sputum?

Yellowish-green sputum is also common in people with cystic fibrosis. Cystic fibrosis is an inherited disease that causes mucus to build up in the lungs and other organs. Brown. This often shows up in people who smoke. It is also a common sign of black lung disease.

Why do you tap your chest?

Your provider may tap you on the chest to help loosen sputum from your lungs.

What is a sputum culture?

A sputum culture is a sample of the gooey substance that often comes up from your chest when you have an infection in your lungs or airways. It is mostly made up of white blood cells that fight infection mixed with germs.

What is a positive sputum sample?

If the sputum sample is abnormal, the results are called “positive.” Identifying the bacteria, fungus, or virus may help diagnose the cause of:

How to prepare for oropharyngeal biopsy?

Patient Preparation: Have patient rinse his/her mouth with water immediately prior to specimen collection. This reduces the number of contaminating oropharyngeal bacteria.

Where is the bronchoscope passed?

Bronchoscope is passed through the mouth or nose directly into the diseased portion of the lung, and sputum or lung tissue is removed. Use to obtain sputum when coughing sputum is not productive or other diagnoses are being considered. • Most expensive and invasive procedure. • Requires special equipment.

Can you cough up sputum without assistance?

Patient coughs up sputum into a sterile container. • Inexpensive. • Easy to do. • Patient may not be able to cough up sputum without assistance or may spit up saliva instead. • Health-care worker has to coach and supervise the patient when collecting sputum. Sputum Induction.

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