How to use the pipette correctly?
Learn how to correctly use a pipette and improve your results instantly:
- Ensure temperature equilibrium: Make sure the pipette, tips and liquids are at room temperature (if experiment allows)
- Maintain consistent pipette angle: Hold the pipette at a consistent angle not exceeding 20 degrees
- How to aspirate: Aspirate by immersing the tips just below the liquid’s surface (2-3 mm)
How to check the accuracy of your pipette?
- Place the micropipette tip against the side of the receiving test tube. Surface tension will help to dispense the contents of the micropipette. ...
- Smoothly depress the plunger to the first stop. Pause, then depress the plunger to the second stop. ...
- Use the tip ejector to discard the tip. ...
How to use a pipette [best practices]?
- Manage Temperature Transfer — Pipettes are very susceptible to thermal transfer from your hands. ...
- Equilibrate Pipette Temperatures — Pipettes are part of a pipetting system. ...
- Protect Against Contamination — Pipettes are very susceptible to contamination, especially the nozzle and internal piston chamber. ...
How accurate are your pipettes?
How to Check the Accuracy of Your Pipette
- Check for Pipette Accuracy Often. Aside from sending your pipette off for calibration every year, your pipettes should be checked for accuracy on a regular basis.
- The Weight of Water. ...
- Pipetting Technique Will Affect Accuracy. ...
- Suck It Up, Eject, and Repeat. ...
- Take Care of Your Valuables. ...
How do you read 1ml pipette?
0:193:23How to Read and Aseptically Handle a Sterile 1 ml Pipette - YouTubeYouTubeStart of suggested clipEnd of suggested clipTo the zero is one milliliter and it's divided into tenths of a milliliter. So from point zero twoMoreTo the zero is one milliliter and it's divided into tenths of a milliliter. So from point zero two point one is 1/10 from point one to point two is another tenth etc.
How do you read a pipette in chemistry?
0:302:43Using Burets and Pipettes - YouTubeYouTubeStart of suggested clipEnd of suggested clipThe marked divisions on both instruments are equal to one milliliter and our smallest at the top andMoreThe marked divisions on both instruments are equal to one milliliter and our smallest at the top and largest at the bottom the unmarked divisions are equal to 1/10 milliliter.
How do you read a 2 mL pipette?
1:552:48Understanding Serological Pipettes - YouTubeYouTubeStart of suggested clipEnd of suggested clipSee how the scale extends past zero at the top to minus one minus two and minus three these extraMoreSee how the scale extends past zero at the top to minus one minus two and minus three these extra graduations.
How do you read a glass pipette?
0:553:39Pipetting: Glass Pipettes - YouTubeYouTubeStart of suggested clipEnd of suggested clipThe number at the top of the pipette shows the maximum volume that can be accurately transferred byMoreThe number at the top of the pipette shows the maximum volume that can be accurately transferred by the pipette. For instance this pipette can transfer a maximum of 10 milliliters with accuracy up to
How do you read a 5 ml pipette?
0:131:13How to Read and Aseptically Handle a Sterile 10 milliliter ml PipetteYouTubeStart of suggested clipEnd of suggested clipTo 9 or from 9 to 8 would be one milliliter. So for example if we wanted to dispense 5 milliliters.MoreTo 9 or from 9 to 8 would be one milliliter. So for example if we wanted to dispense 5 milliliters. We would draw it up to the 5.
How do you use a pipette correctly?
1:593:10a short step-by-step introduction into proper pipetting - YouTubeYouTubeStart of suggested clipEnd of suggested clipWhen expecting liquid the angle of the pipette should be between 20 and 45 degrees whilst the tipMoreWhen expecting liquid the angle of the pipette should be between 20 and 45 degrees whilst the tip should stay in contact with the vessel. Wall. This guarantees optimal flow out of the liquid.
How do you take 0.1 ml in a micropipette?
2:543:38How to Use a Micropipette - YouTubeYouTubeStart of suggested clipEnd of suggested clipAs you push down on the plunger. The fluid in the tip will be pushed out into the tube. RememberMoreAs you push down on the plunger. The fluid in the tip will be pushed out into the tube. Remember that the plunger has two stops.
How do you measure volume using a pipette?
0:492:07Measuring Liquids with an Adjustable Volume Micropipette - YouTubeYouTubeStart of suggested clipEnd of suggested clipIn general twisting the plunger clockwise reduces the volume and twisting the plungerMoreIn general twisting the plunger clockwise reduces the volume and twisting the plunger counterclockwise increases the volume.
How do you use a 5ml pipette?
1:064:25How to Use a Pipette - YouTubeYouTubeStart of suggested clipEnd of suggested clipSo as you reach the top of this bulb section slowly pull the rest of the way. Out. So the volumesMoreSo as you reach the top of this bulb section slowly pull the rest of the way. Out. So the volumes going up the bulb.
How do you read a burette and pipette?
0:021:01How to Read a Buret in Chemistry - YouTubeYouTubeStart of suggested clipEnd of suggested clipHere we have a 50ml burette and unlike other volumetric instruments such as the graduated cylinderMoreHere we have a 50ml burette and unlike other volumetric instruments such as the graduated cylinder burette SAR read by reading the empty space above the liquid.
What are the units of markings on the pipette?
The lower portion of the stem is divided by marks into two equal parts – 0.5 and 1, totaling one unit.
How do you use a 25 ml pipette?
0:294:15Using a volumetric pipette correctly - YouTubeYouTubeStart of suggested clipEnd of suggested clipAnd the tip of the pipette is below the surface of the liquid scroll. The wheel downwards to drawMoreAnd the tip of the pipette is below the surface of the liquid scroll. The wheel downwards to draw the solution into the pipette. The button releases the liquid.
How do you measure volume using a pipette?
0:492:07Measuring Liquids with an Adjustable Volume Micropipette - YouTubeYouTubeStart of suggested clipEnd of suggested clipIn general twisting the plunger clockwise reduces the volume and twisting the plungerMoreIn general twisting the plunger clockwise reduces the volume and twisting the plunger counterclockwise increases the volume.
How do you use a pipette to deliver a specific volume of a liquid?
Holding the top of the pipette tightly with your finger, bring the pipette to the flask where the liquid is to be delivered and again allow tiny amounts of air into the top of the pipette in order to slowly drain the liquid to the desired mark (Figure 1.25b; Figure 1.25c shows the delivered volume to be slightly below ...
How do you draw liquid into a pipette?
0:564:25How to Use a Pipette - YouTubeYouTubeStart of suggested clipEnd of suggested clipAnd the other is the outlet valve we push this and air is just allowed to come in this is similar toMoreAnd the other is the outlet valve we push this and air is just allowed to come in this is similar to letting your thumb off of a straw. And the fluid follows. Out. So let's use the volumetric pipette.
How do you carry out the pipetting technique using a volumetric pipette?
Place the tip of the pipet into the receiving vessel and remove your finger to allow the liquid to drain. Touch the pipet once to the side of the vessel. There will be a small amount of liquid in the tip. Most pipets are calibrated to account for this liquid.
How to use a pipette to measure volume?
To use this technique, press the plunger to first stop and slightly submerge the pipette tip into the liquid, aspirate your measured volume by releasing the plunger slowly to prevent bubbles. To dispense the liquid, place the tip against the side of the receptacle, then slowly press the plunger through the first stop to the final blow out position while ‘touching off’ the last drop from the tip.
How to take a sample of liquid from a pipette?
Place the pipette tip against the wall of the receptacle and press the plunger to the first stop, holding the plunger in place and remove the tip from the receptacle. You will now have a sample of liquid that remains in the tip, but is not part of the measurement. You can then repeat the aspiration procedure and continue.
How to release plunger tip?
Move the end of the tip away from the liquid and release the plunger to the rest position.
How to use a reverse pipette?
To use this technique, press plunger all the way through to the third stop position (all the way down), slightly submerge into liquid, and slowly release the plunger all the way back to the top and aspirate the liquid into the tip. Place the pipette tip against the wall of the receptacle and press the plunger to the first stop, holding the plunger in place and remove the tip from the receptacle. You will now have a sample of liquid that remains in the tip, but is not part of the measurement. You can then repeat the aspiration procedure and continue.
How many grades of pipette are there?
Within pipette calibration there are five widely used grades of pipettes, all of which have specific guidelines and requirements regarding use, testing, maintenance, and measurement. The five grades of pipettes include disposable/transfer, graduated/serological, single-channel, multichannel, and repeat pipette. From the most basic transfer pipette dropper to the advanced repeat dispensing pipettor, the manner in which the equipment is handled will impact the accuracy of the test results.
What is a single channel pipette?
A single-channel pipette is a non-disposable instrument, usually an air-displacement design which produces accurate measurement results with the use of one disposable tip. There are two common techniques associated with single-channel pipetting:
How to fill a syringe tip?
Slide the filling lever down as far as it goes. Raise the locking clamp upward. Insert the syringe-type tip into the barrel so that it clicks into position, then close the lever. Immerse the tip into the liquid at a 90 degree angle. Slowly slide the filling lever upward to fill the tip completely.
How to use a pipette?
The first step when using a pipette is setting the pipette to your desired volume. To do this, locate the small window on your pipette that has three numbers in it. If your pipette has a lock/unlock switch, switch it to the unlock position. Then, turning the plunger clockwise or counter-clockwise until you reach the desired amount.
What is a pipette in science?
2. Introduction: How to Pipette. Using a pipette is an essential part of working in a science laboratory. Pipettes, specifically micro-pipettes, are used for accurately measuring and transferring small amounts of solutions from on container to another.
How to get rid of a pipette tip?
To do this, press down on the tip ejector button, which is a small button usually next to the plunger, while holding the pipette over a waste bucket. This will move a metal or plastic arm down, forcing the tip off of the pipette. It is always a good idea to change tips when you are changing solutions, or if it touches any surface; these both reduce risks of contamination.#N#Congratulations, you made it! You have successfully learned how to pipette. Now that you know this essential science skill, a world of pipetting possibility awaits!
How to remove a pipette from a container?
Slowly push the plunger down all the way to the first stop, then the second stop to make sure all solution has been pushed out of the tip. Once done, remove the pipette from the container and close the lid.
How many uL is a P20 pipette?
Although each pipette brand varies slightly, micro-pipettes generally come in three sizes; the P20 which can pipette from 2-20 uL, the P200 which can pipette volumes from 20-200, and the P1000, which - you guessed it - can pipette volumes ranging from 200-1000 uL or 1 mL. Continue reading for step-by-step instructions on how to use a pipette.
What is the first stop on a pipette?
The first stop is the stop that changes; it is the amount that you set the pipette to in the previous step, and it is shown by the first picture. The second stop, shown in the second picture, is an extra 'push' that it sometimes needed to remove all of the liquid from the tip.
What do you need to pipette?
To pipette, you will need:#N#-A pipette#N#-A box of tips#N#-A container holding the solution you wish to pipette#N#-An empty container for the solution (s) to be transferred into#N#-A clean working space#N#-A waste bin#N#-Gloves if you don't like your hands getting dirty or you are working with potentially hazardous materials
How to get accurate results with a pipette?
To get accurate results, calibrate the pipette with the volatile compound you want to pipette. If you use air displacement pipettes, aspirate and dispense the liquid a few times keeping the tip in the liquid. By doing so, the air inside the pipette will be saturated with vapor of the volatile compound. 2.)
How to make a pipette more accurate?
Hold the pipette so the "grippy finger rest" rests on your index finger. To maximize accuracy, the pipette, tip and liquid should be at the same temperature. Check that you are using tips recommended for this pipette. To ensure accuracy, use only high-quality tips made from contamination-free polypropylene.
How to prevent aerosols from entering a pipette?
Samples or aerosols from samples can enter the cone of the pipette. Prevention: Keep the pipette vertical when pipetting in order to prevent liquid from running into the pipette body. Release the push-button slowly. To avoid aerosol contamination, use filter tips or use a positive displacement pipette and tips.
What is reverse pipetting?
The reverse technique is used for pipetting solutions with a high viscosity or a tendency to foam. Reverse pipetting is only possible with air displacement pipettes
What is the most important factor in measuring a pipette?
One of the most important factors in accurate Pipette measurement is the Tip. The Pipette and the Tip form a System. The best Pipetting results are naturally achieved by those manufactured together to ensure complete compatibility between Pipettors and Pipettor Tip.
What is the most important factor in pipetting accuracy?
Temperature. The most important factor in pipetting accuracy is the liquid temperature . The figure below shows the change in volume when the liquid has a different temperature than the pipette and air. If the temperature of the liquid, pipette and air is the same, the accuracy is not significantly affected. Density.
How to pipette whole blood?
Pipetting whole blood. Use forward technique steps 1 and 2 to fill the tip with blood (do not pre-rinse the tip). Wipe the tip carefully with a dry clean cloth. Dip the tip into the blood and press the operating button to the first stop. Make sure the tip is sufficiently below the surface.