Procedure or Protocol of Streak Plate Method
- Quadrant Streaking Procedure. Lift the Petri plate in your left hand and hold it at an angle of 60°. The sample is...
- T-Streaking Procedure. The sample is spread over about 1/3 rd of the media in the Petri plate from the rim to the center...
- Continuous Streaking Procedure. Place the loop at one end of the plate and start...
What are the steps of streak plate method?
Three Sector Streak (t streak):
- Sterilize the wire loop.
- Cool the loop by touching it on the edge of the sterile agar plate.
- Dip the loop into the broth culture containing the mixture of bacteria.
- Lift the lid of the plate just enough to insert the loop. ...
- The loop has picked up thousands of bacteria which are spread out over the surface of the agar.
- Sterilize the loop in the flame.
What are the advantages of streak plate method?
What are the advantages of streak plate method? Streak plating is a method that allows microorganisms to be grown from a sample on an agar plate. The agar provides the nutrients needed by the organism (s) being grown. Obligate anaerobes require a complete lack of oxygen to thrive, and the streak plate method is aerobic.
What is the goal of streak plate technique?
What is the purpose of the streak plate technique? The purpose of the streak plate method is to produce an isolated colony of an organism on the agar plate. Isolation of the organism is a must in a mixed culture, especially if you need to thoroughly study the colony morphology of a particular organism.
What is the streak plate technique?
What are the Similarities Between Streak Plate and Spread Plate?
- Streak plate and spread plate are two microbial techniques that we use in bacteriology.
- Both methods are in use to isolate or separate bacteria from a mixture.
- In both techniques, we can dilute the sample prior to inoculation.
- Both methods require solidified agar plates.
- Bacteria grow on the surface of the medium in both methods.
How do you do a streak plate test?
Immediately streak the inoculating loop very gently over a quarter of the plate using a back and forth motion (see area 1 in the figure above). Flame the loop again and allow it to cool. Going back to the edge of area 1 that you just streaked, extend the streaks into the second quarter of the plate (area 2).
What tool is used to streak a plate?
The streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop. The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria.
How do you make a streak plate at home?
3:396:47Your Home Yeast Lab Made Easy - Streak Plates - YouTubeYouTubeStart of suggested clipEnd of suggested clipSo you take your first sample of yeast. And you streak across the top of the plate forming a smallMoreSo you take your first sample of yeast. And you streak across the top of the plate forming a small number of streaks. You would then flame your loop cross. Back over your first set of streaks.
What is simple streaking?
In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.
Why streak plate method is used?
Agar streak plates are an essential tool in microbiology. They allow bacteria and fungi to grow on a semi-solid surface to produce discrete colonies. These colonies can be used to help identify the organism, purify the strain free of contaminants, and produce a pure genetic clone.
What is an inoculating loop used for?
An inoculation loop, also known as a smear loop, inoculation rod or microstreaker, is a simple tool used primarily by microbiologists to take and transfer a small sample (inoculum) of a microorganism culture, for example for stripping on a culture plate.
What is a ceramic streak plate?
An unglazed piece of porcelain, such as a tile, used to test the characteristic streak of minerals by rubbing the mineral across the tile.
How do you streak an agar slant?
0:401:26How to Inoculate a Slant - MCCC Microbiology - YouTubeYouTubeStart of suggested clipEnd of suggested clipOpen up the cap of the tube that you'd like to inoculate. Take the inoculating loop. Put it on theMoreOpen up the cap of the tube that you'd like to inoculate. Take the inoculating loop. Put it on the bottom of the slant on the surface street get back and forth.
Introduction
If you have a glycerol stock or stab culture of bacteria and need to purify plasmid DNA from it, you will want to isolate an individual clonal population (single colony) of bacteria from this stock.
Protocol Video
Watch the protocol video below to learn how to isolate single bacterial colonies.
What is Streak Plate Method ?
Streaking is a method that isolates a pure strain from a species of bacteria. A sample is taken from a colony and a microbiological culture is grown on the new plate in order for the organism to be identified properly.
What is the objective of the streak plate method?
The objective of streak plate method is to obtain isolated microbial colonies by creating areas of dilution on the agar petri plate. (3
What is the principle of the streak plate method?
The streak plate method requires the number of organisms in the inoculums be reduced. The procedure includes a dilution technique which requires spreading a loopful of culture over the agar plate surface.
What is the purpose of the streak plate technique?
The purpose of the streak plate method is to produce an isolated colony of an organism on the agar plate. Isolation of the organism is a must in a mixed culture, especially if you need to thoroughly study the colony morphology of a particular organism. (4, 7)
How is the streak plate method done?
The inoculating loop should be sterilized in the Bunsen burner by simply putting the loop into the flame. Wait for the loop to turn red indicating that it is already hot. Let it cool down for a few minutes.
Streak plate method advantages and disadvantages
The streak plate method enables you to select and work with individual colonies.
What is the Streak Test?
The "streak test" is a method used to determine the color of a mineral in powdered form. The color of a mineral's powder is often a very important property for identifying the mineral.
How to Conduct the Streak Test
The streak test should be done on clean, unweathered, or freshly broken specimens of the mineral. This is done to reduce the possibility that a contaminant, weathered coating, or tarnish will influence the results of the test.
Don't Be Wimpy!
The most common error made by people who are doing the streak test for the first time is to lightly rub the specimen back and forth on the surface of the streak plate. This will not produce a proper streak. Some mineral specimens are so hard that very firm pressure and determination are required to produce a mineral powder.
Why Use the Streak Test?
The streak test is valuable because many minerals occur in a variety of apparent colors - but all specimens of that mineral share a similar streak color. For example: specimens of hematite can be black, red, brown, or silver in color and occur in a wide variety of habits; however, all specimens of hematite produce a streak with a reddish color.
Don't Be Deceived!
A number of things can cause a streak test to give unreliable results. To avoid problems, keep the following items in mind.
Refreshing Your Streak Plate
Streak plates that have been used heavily will be covered with streaks and powdered mineral. They can easily be cleaned with water and a piece of wet or dry 220 grit sandpaper. Aluminum oxide or silicon carbide sandpaper works best because the granules are hard enough to smooth the surface of the streak plate.
Other Uses for Streak Plates
In addition to their use in doing the streak test, streak plates can be used any time you need a small amount of powdered mineral. In doing the acid test to distinguish calcite from dolomite, dolomite might require being powdered to show effervescence with dilute hydrochloric acid.
