How is Janus Green used to visualize living cells? Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits. Click to see full answer.
How can I stain a specific cell line with Janus green stain?
Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes.
How does Janus Green B stain the mitochondria?
By staining the celery molecules with Janus Green B, it is possible to watch this enzymatic reaction. The stain will be drawn into the mitochondria and turn them greenish blue. If the dye looses hydrogen, however, the dye loses color, showing what mitochondria does to food (sugar) molecules that enter the organelle.
What is Janus Green B?
Janus Green B belongs to the phenazine group of dyes. It is a dark green/dark brown/dark black powder. Janus Green B should be oxidized to become colored.
What is Janus green stain used for in Elisa?
Find out more. Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622). Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain.
How does Janus green work?
The indicator Janus Green B changes colour according to the amount of oxygen present. When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.
Why is Janus Green B used in this experiment?
Janus green B (JG-B) dye is used for vital staining of mitochondria and its reduction and oxidation shows the electron transfer chain alteration. The defect in electron transfer chain of mitochondria by paraquat is linked to free radical formation.
What is the role of Janus green stain in the slide preparation?
Answer: Janus green is used to stain exclusively mitochondria. If this stain is not used, mitochondria can't be distinguished from other cellular organelles.
Which stain is used to visualize mitochondria?
Janus greenJanus green is a super vital stain which is used to demonstrate that mitochondria are oxidation-reduction sites in the cell.
How does Janus green stain mitochondria?
Janus Green B is chemically diethyl safranin-azo-dimethyl aniline. It is oxidized to become colored. The cytochrome c oxidase helps to maintain the dye in an oxidised state in the mitochondria. In its oxidized state it is bluish green in colour.
Which organism is observed by using Janus Green B in the laboratory?
Mitochondria may be observed in living cells with the aid of a vital stain called Janus Green B. This stain is colored when it is an oxidizing agent.
What is the meaning of Janus green?
Definition of Janus green : a basic azine dye used especially as a vital biological stain (as for mitochondria)
Which stain is useful in observing mitochondria under the light microscope?
Janus green is a basic dye and vital stain used for staining the mitochondria of a cell.
How do you make Janus green solution?
To prepare a Janus Green B solution, use a 1% aqueous solution. Add 1 g of Janus Green B to 75 ml of DI water. Dilute to a final volume of 100 ml with DI water.
Which colour developed during staining of mitochondria?
The Janus green is a basic dye which is also used as a vital stain in histology. This dye is used for staining the mitochondria.
What does fast green stain?
fast green A green dye used in optical microscopy that stains cellulose, cytoplasm, collagen, and mucus green. It is frequently used to stain plant tissues, with safranin as a counterstain. Unlike light green, a similar dye, it does not fade easily.
Which colour develops during staining of mitochondria *?
Complete answer: Safranin stain is used to stain and observe the organelle mitochondria in an onion peel. Safranin stain is a pink colored biological stain which is mostly used for staining purposes in biological experiments. Safranin stain helps by developing the red color of the nuclei of the cell.
Applications
Our Abpromise guarantee covers the use of ab111622 in the following tested applications.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
Publishing research using ab111622? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
For this janus green assay, the protocol states to read the plate at 595nM. Can the absorbance of the 0.1 ml HCl solution be read in the same wells the cells were grown or do you recommend spinning down the plate, removing the solution to clean well prior to taking OD measurements?
Description
Janus Green B has been used in staining technique to determine cell density, to detect the presence of telocytes in trachea and lungs and used as a dye to visualize bonghan ducts inside lymphatic vessels of rabbits.
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