What is Diff Quik stain made of?
The Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying.
How do you use Diff Quick fixative?
Fix in “Diff Quick” Fixative (or methanol) for 30 secs/drain 3. Stain with “Diff Quick” solution II for 30 secs/drain 4. Counterstain (optional) with “Diff Quick” solution I for 30 secs/drain 5. Rinse in tap water to remove excess stain 6. Rapidly dehydrate in absolute alcohol 7. Clear and mount
What is the difference between Romanowski stain and Diff Quick stain?
"Diff-Quick" is a proprietory brand of a Romanowski stain. The Romanowski group of stains are defined as being the black precipitate formed from the addition of aqueous solutions of methylene blue and eosin, dissolved in methanol.
How long do you soak slides before staining?
Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying. Some specimens, e.g. lymph node aspirates, may be under-stained with this standard technique.
How do you clean Diff-Quik stains?
Rinse the slide with the rinse buffer solution or under gently running tap water until the slide is pink and the water runs clear. Rest the slide vertically and allow it to air-dry. Do not wipe or blot the top of the slide with tissue or you will remove or destroy the cells.
How do you use Diff-Quik stain?
MethodAllow smears to dry.Dip slide or tape-strip five times, for one second each, into Fixative. ... Dip slide or tape-strip five times, for one second each, into Stain 1. ... Dip slide or tape-strip five times, for one second each, into Stain 2. ... Rinse slide or tape-strip in distilled water or Weise's buffer, pH 7.2.More items...
What are the 3 Diff-Quik stains?
The Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue).
What does Diff-Quik stain identify?
Diff Quik staining method for detection and identification of monosodium urate and calcium pyrophosphate crystals in synovial fluids | Annals of the Rheumatic Diseases.
Is Diff Quick toxic?
Hazard statements : Diff Quik Fixative Solution H225 - Highly flammable liquid and vapor. H331 - Toxic if inhaled.
Is Diff-Quik a Gram stain?
Diff-Quik stain, a variant of Romanowsky stain, is used to quickly identify cells and bacteria. However, it does not differentiate between gram-positive or gram-negative bacteria.
What is Diff-Quik fixative solution?
RAL Diff-Quik fixative solution It fixes air-dried blood smears. This methanol based solution will stabilize cellular components. Solution I and II are applied individually to the fixed smear to differentially stain specific cellular components.
How do vets stain slides?
The staining procedure: Make sure the sample on the slide is dry. Dip the slide in each jar between six-10 times (10-15 seconds in each solution). ... Wash the slide with water after jar number 3 ONLY. Dip the slide in the water jar. Never wash the slide in between the staining process. Alternative to Jar 4:More items...
Why should the smear be rinsed with tap water?
You want to wash your slide before blocking them to get rid out of the acid and not over-digesting. Tap water has a nice turbulence which make a very efficient washing step.
What cells are visible with Diff Quick staining?
ResultsStructureColourErythrocytesPink/yellowish redPlateletsViolet/purple granulesNeutrophilsBlue nucleus, pink cytoplasm, violet granulesEosinophilsBlue nucleus, blue cytoplasm, red granules5 more rows
What could be the cause of a poorly stained blood preparation?
Drop of blood too large or too small. Spreader slide pushed across the horizontal slide in a jerky manner. Failure to keep the entire edge of the spreader slide against the horizontal slide while making the smear. Failure in using appropriate angle for the spreader slide.
What does New methylene blue stain?
New Methylene Blue (NMB) is a supravital stain which when mixed with whole blood, stains RNA and DNA deep blue. Reticulocyte count: RNA in cytosol of immature RBC is precipitated into strands and clumps, staining deep blue when mixed with NMB.
What is diff quick stain?
Diff-Quick (Diff-Quik) Staining Protocol. "Diff-Quick" is a proprietory brand of a Romanowski stain. The Romanowski group of stains are defined as being the black precipitate formed from the addition of aqueous solutions of methylene blue and eosin, dissolved in methanol.
What is the Romanowski group?
The Romanowski group of stains are defined as being the black precipitate formed from the addition of aqueous solutions of methylene blue and eosin, dissolved in methanol. The variants of the Romanowski group differ in the degree of oxidation (polychroming) of the methylene blue stain prior to the precipitation.
What is the stain class?
The stain class was originally designed to incorporate cytoplasmic (pink) staining with nuclear (blue) staining and fixation as a single step for smears and thin films of tissue (spread preparations of omentum). Minor modifications of working stain concentration and staining time have been made over the years for fixed tissue sections.
How long to fix diff quick fixative?
2. Fix in “Diff Quick” Fixative (or methanol) for 30 secs/drain
Where can I buy diff quick?
1. “Diff Quick” solution I/II or kits can be purchased from IHC Store
