Pipette
A pipette (sometimes spelled pipet) or dropper is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and pr…
Why are pipette tips used?
Filtered pipette tips help to reduce the risk of aerosols forming in the laboratory. They also protect the pipette shafts from contamination and reduce the risk of cross contamination. These pipette tips are often used in contamination sensitive applications such as forensics and clinical diagnostics.
How are pipette tips made?
How are pipette tips manufactured?Injection molding of a pipette tip in a mold.Opening of the mold and drawing the plastic section into a capillary.The unsolidified plastic section is selectively heated.The tip is drawn vertically, and the tip is shaped using a molding element.
What are pipette filter tips?
A filter tip is beneficial when the assay is sensitive to cross-contamination, or if the sample can contaminate the lower part of the pipette. The filter prevents liquid from accidentally splashing the inside of the pipette, and reduces aerosols from penetrating the pipette tip cone during pipetting.
What material are pipette tips?
polypropyleneWhat are pipette tips? Pipette tips are single-use laboratory consumables made from polypropylene and are also available with an aerosol barrier filter made of polyethylene.
Are pipette tips plastic?
Most pipette tips and their boxes are made of either low density polyethylene (LDPE, plastic #4), or polypropylene (PP, plastic #5).
Are pipette tips reusable?
Yes. Conical tubes and even pipette tips can be washed and/or autoclaved and reused. Pipette tip boxes can be repurposed to hold other lab supplies or as containers for Western blots.
What is the tip of a pipette called?
The major difference is that the disposable tip is a microsyringe (plastic), composed of a capillary and a piston (movable inner part) which directly displaces the liquid.
Whats the difference between tips and filters?
Cigarette Filter - These are ripped off cigarettes or bought in packs from tobacco shops. They are often used for smoking spliffs (tobacco mixed with cannabis). Glass Tips Joints - These are inserted on the end of a blunt or joint.
What are the 3 types of pipettes?
The five grades of pipettes include disposable/transfer, graduated/serological, single-channel, multichannel, and repeat pipette.
Are pipette tips sharps?
Plastic pipettes and pipette tips are a special type of sharp since they can puncture or cut under special circumstances. The expanded sharps can be called pseudo sharps.
What are the 4 parts of the pipette?
Components of a micropipette: These include the plunger, digital display, tip cone, tip ejector and grippy.
Are pipette tips biodegradable?
Neptune features stackable pipette tips that reduce packaging waste up to 90% and take up to 80% less storage space. Friendly Green makes an eco-friendlier choice for industrial wipes comparable to Kimberly Wipes. They're made from at least 80% post-consumer waste and are biodegradable.
How are plastic pipettes made?
Plastic Pasteur pipettes, also referred to as transfer pipettes, have their stems and bulbs in the form of a single piece of moulded plastic. There are many generic and brand names on the market, but they are all manufactured by the blow moulding process.
How do you make a pipette?
1:593:10a short step-by-step introduction into proper pipetting - YouTubeYouTubeStart of suggested clipEnd of suggested clipWhen expecting liquid the angle of the pipette should be between 20 and 45 degrees whilst the tipMoreWhen expecting liquid the angle of the pipette should be between 20 and 45 degrees whilst the tip should stay in contact with the vessel. Wall. This guarantees optimal flow out of the liquid.
What is the tip of a pipette called?
The major difference is that the disposable tip is a microsyringe (plastic), composed of a capillary and a piston (movable inner part) which directly displaces the liquid.
Are pipette tips sharps?
Plastic pipettes and pipette tips are a special type of sharp since they can puncture or cut under special circumstances. The expanded sharps can be called pseudo sharps.
What are pipette tips?
Pipette tips are single-use laboratory consumables made from polypropylene and are also available with an aerosol barrier filter made of polyethylene.
Are pipette tips reusable?
Pipette tips can be used to measure liquid multiple times but once ejected from pipette they should not be reused. To achieve a leakage free seal w...
When should you change pipette tips?
Generally it is recommended that pipette tips should be changed between different liquids and samples but with specific liquid properties like when...
What are the types and functions of different pipette tips?
There are multiple types of pipette tips with specific functions: - standard tips are suitable for most applications, - filtered pipette tips are...
How do you use a pipette tip?
You should use the pipette manufacturers recommended pipette tips to achieve best possible performance. The pipette tips should be picked directly...
Can I cut the end of a pipette tip to make it wider?
Never cut, bend or otherwise manipulate the pipette tips as it can affect your pipetting results. Sartorius offers wide bore pipette tips when samp...
How are pipette tips sterilized?
Sartorius non-filtered pipette tips can be sterilized in tip trays by autoclaving at 121°C, 1 bar (252°F, 15 p.s.i.) for 20 minutes. You can also p...
Why do we use disposable tips for pipetting?
Disposable pipette tips are a cost efficient method for measuring reagents and samples. They are easy-to-use and effective in helping prevent conta...
How to choose the right pipette tip for your experiment?
First determine whether you need sterile pipette tips. In applications where protecting samples from cross contamination is critical consider using...
What are pipette tips?
Pipette tips fit on the end of a pipette, creating an air-tight seal. Commonly made from polypropylene, they are made for one-time use and are avai...
What is BioClean Ultra?
BioClean refers to the exceptionally high standard to which all Rainin air-displacement tips are tested and found to be free of detectable amounts...
What is pipette tip sterility?
Pipette tip sterility refers to the absence of bacteria or any living microorganisms or spore, but without an additional qualifier (such as BioClea...
What are pipette tips?
Pipette tips are single-use laboratory consumables made from polypropylene and are also available with an aerosol barrier filter made of polyethylene.
How to choose the right pipette tip for your experiment?
First determine whether you need sterile pipette tips. In applications where protecting samples from cross contamination is critical consider using filtered pipette tips. Consider the characteristics of the liquids you are working with: for aquous liquids use standard tips and for viscous liquids or when you want to preserve expensive reagents or precious samples use low retention tips.
When should you change pipette tips?
Generally it is recommended that pipette tips should be changed between different liquids and samples but with specific liquid properties like when pipetting warm or cold liquids best results are achieved by changing the tip after each dispensing. To learn more about best pipetting practices read more about Sartorius Pipetting Academy trainings. See our Pipetting Academy offering at https://www.sartorius.com/en/knowledge/trainings/pipetting-academy
How are pipette tips sterilized?
Sartorius non-filtered pipette tips can be sterilized in tip trays by autoclaving at 121°C, 1 bar (252°F, 15 p.s.i.) for 20 minutes. You can also purchase ready-to-use sterilized pipette tips. Commercial sterilization is regularly done by irradiation or with ethylene oxide.
Why do we use disposable tips for pipetting?
Disposable pipette tips are a cost efficient method for measuring reagents and samples. They are easy-to-use and effective in helping prevent contamination.
Should I use self-sealing filter tips?
Self-sealing filter tips block liquid flow in case of over-pipetting but also retain your sample. Sartorius Safetyspace ® filter tips have the unique Safetyspace ® gap which leaves plenty of space between the maximum volume of the tip and the filter in reverse pipetting, so you don't have to worry about over-pipetting.
When should I use low retention tips ?
Low retention pipettes tips are especially advantageous when pipetting solutions with detergents, such as PCR master mixes, enzyme solutions and buffers, and viscous liquids. Low Retention tips have lower residual liquid after dispensing which saves your precious samples and reagents.
What is the job of a pipette?
Pipetting viscous or volatile liquids, repeat dispensing, transferring aggressive liquids safely or serological pipetting – these are tasks that are best served by our specialized pipettes and tips.
What are rainin tips made of?
Made from 100% virgin polypropylene in a fully automated, Class 100,000 clean room facility, Rainin tips provide excellent clarity and flexibility for accurate touch-off. To assure absolute purity, no additives or dyes are used, and we follow ISO 9001-compliant manufacturing procedures to eliminate all external sources of contamination.
What is PureSpeed Protein Tips?
PureSpeed Protein Tips offer a faster, more convenient and reliable purification workflow for obtaining highly concentrated and pure functional proteins. The result: higher productivity, less waste and reduced risk.
What is the most economical way to get Bioclean tips?
Buying in bulk is the most economical way to get BioClean quality tips.
What is a rainin litetouch?
Unique to Rainin, the LiteTouch System connects a specially shaped nozzle to a perfectly matched LTS tip to deliver the industry's lowest tip mounting and ejection forces. The result: fewer repetitive stress injuries and more comfort while pipetting.
How long does it take to purify a sample?
Purify samples in as little as 15 minutes.
Why use hydrophobic filters?
Hydrophobic filters to prevent cross contamination from aerosols and liquids.
What are Hamilton tips made of?
Hamilton tips are made from chemically resistant, virgin polypropylene for excellent clarity and smooth surfaces. All AdvanTip products are certified to be safe from RNase, DNase, and pyrogens. Hamilton pipette tips are universal-fit.
Can Hamilton pipettes be autoclaved?
Yes, the Hamilton adjustable volume, fixed volume and multi-channel pipettes are autoclavable at 121°C without the need of disassembly or recalibration.
Can you autoclave Hamilton pipette?
It is suggested to loosen the lower body of the Hamilton pipette and placing it inside an autoclave bag before autoclaving. Doing so may extend the life of the pipette.
ClipTip Pipette Tips
Thermo Fisher Scientific has been providing innovative liquid handling solutions to laboratories like yours for decades. Rely on our expertise and innovation for all your pipetting needs.
SoftFit-L Tips
The SoftFit-L Tip was designed for optimal performance with Rainin™ LTS pipettes. SoftFit-L Tips deliver the accuracy and precision you expect and maintain the ergonomic benefits of the pipette with a positive-stop collar that reduces tip attachment and ejection forces.
Pipettes and pipette tips selection
Lightweight, ergonomic single channel pipettes available in both fixed or variable volume models for accurate and precise pipetting.
ClipTip pipetting systems
Worry-free pipetting is here with the only tip that clips onto your pipette with a light touch, and locks tips firmly in place so they will not loosen or fall off, regardless of application pressure.
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