
Biological indicators (BIs), as defined by ANSI
American National Standards Institute
The American National Standards Institute is a private non-profit organization that oversees the development of voluntary consensus standards for products, services, processes, systems, and personnel in the United States. The organization also coordinates U.S. standards with internation…
What are examples of biological indicators?
Microbial indicators
- Chemical pollutants. Microorganisms can be used as indicators of aquatic or terrestrial ecosystem health. ...
- In oil and gas exploration. Microbial Prospecting for oil and gas (MPOG) is often used to identify prospective areas for oil and gas occurrences.
- Microalgae in water quality. ...
What is the definition of indicator in biology?
Definition of Biological indicator. They are any biological species or group of species whose function, population, or status can reveal the qualitative status of the environment. One example of a group of bio-indicators are the copepods and other small water crustaceans that are present in many water bodies.
What do the different indicators mean?
Indicators are clues, signs or markers that measure one aspect of a program and show how close a program is to its desired path and outcomes. Indicators are realistic and measurable criteria of project progress. They should be defined before the project starts, and allow us to monitor or evaluate whether a project does what it said it would do.
What is positive biological indicator?
A biological indicator is called positive when: a) the incubation process is complete. b) there is no growth in the ampule after incubation. c) there is growth in the ampule after incubation. d) prior to sterilization. c) there is growth in the ampule after incubation.

What are biological indicators?
Bioindicators are living organisms such as plants, planktons, animals, and microbes, which are utilized to screen the health of the natural ecosystem in the environment. They are used for assessing environmental health and biogeographic changes taking place in the environment.
What is a biological indicator and what is the purpose of the indicator?
A biological indicator provides information on whether necessary conditions were met to kill a specified number of microorganisms for a given sterilization process, providing a level of confidence in the process. Endospores, or bacterial spores, are the microorganisms primarily used in BIs.
What does a positive biological indicator mean?
On the other hand, a positive BI indicates a sterilization process failure that may mean that there are still living and potentially infection-causing microorganisms on the medical devices. Biological indicator resistance - the critical aspect of BI performance.
What is a biological indicator test?
Biological indicators, or spore tests, are the most accepted means of monitoring sterilization because they assess the sterilization process directly by killing known highly resistant microorganisms (e.g., Geobacillus or Bacillus species).
What is the concern if the biological indicators have a negative reading?
The results of a "negative" test indicate sterilization. Failure to kill the spores (a "positive" test, bacterial growth) is a significant event that requires immediate action.
What are the 3 types of sterilization?
Types of SterilizationSteam Sterilization.Dry Heat Sterilization.Ethylene Oxide (EtO) Sterilization.
What is a biological indicators for sterilization?
Biological indicators (BIs) provide information on whether necessary conditions were met to kill a specified number of microorganisms (bacterial spores) for a given sterilization process.
What should you do if a spore test comes back as failed?
3:204:19Failed Spore Test - What do you do? - YouTubeYouTubeStart of suggested clipEnd of suggested clipBefore you can put that sterilizer. Back into service after the second consecutive failure. The CDCMoreBefore you can put that sterilizer. Back into service after the second consecutive failure. The CDC requires that you do three consecutive biological monitoring tests that pass.
What is the difference between chemical indicator and biological indicator?
A biological indicator is used to ensure the chamber of your autoclave is getting completely saturated in the sterilization process. And the Chemical Indicators are used to be certain the steam is covering the entire load, and that the instruments are receiving the necessary amount of steam for proper sterilization.
What causes sterilization failure?
What causes sterilization failures ? Improper contact of sterilizing agent, improper temperature, and overloading of the sterilizer.
What is a bioindicator?
A bioindicator is an organism or biological response that reveals the presence of pollutants by the occurrence of typical symptoms or measurable responses and is , therefore, more qualitative. These organisms (or communities of organisms) can be used to deliver information on alterations in the environment or the quantity ...
What is biomonitor in biology?
This refers to the measurement of specific properties of an organism to obtain information on the surrounding physical and chemical environment. Bioaccumulative indicators are frequently regarded as biomonitors.
Why are biomonitors important?
The importance and relevance of biomonitors, rather than man-made equipment, are justified by the observation that the best indicator of the status of a species or system is itself . Bioindicators can reveal indirect biotic effects of pollutants when many physical or chemical measurements cannot. Through bioindicators, scientists need ...
Why are amphibians bioindicators?
Amphibians, particularly anurans (frogs and toads), are increasingly used as bioindicators of contaminant accumulation in pollution studies. Anurans absorb toxic chemicals through their skin and their larval gill membranes and are sensitive to alterations in their environment. They have a poor ability to detoxify pesticides that are absorbed, inhaled, or ingested by eating contaminated food. This allows residues, especially of organochlorine pesticides, to accumulate in their systems. They also have permeable skin that can easily absorb toxic chemicals, making them a model organism for assessing the effects of environmental factors that may cause the declines of the amphibian population. These factors allow them to be used as bioindicator organisms to follow changes in their habitats and in ecotoxicological studies due to humans increasing demands on the environment.
What is the purpose of a biomonitor?
The use of a biomonitor is described as biological monitoring and is the use of the properties of an organism to obtain information on certain aspects of the biosphere. Biomonitoring of air pollutants can be passive or active. Experts use passive methods to observe plants growing naturally within the area of interest.
Why is knowledge and control of environmental agents important?
Knowledge and control of environmental agents is essential for sustaining the health of ecosystems. Anurans are increasingly utilized as bioindicator organisms in pollution studies, such as studying the effects of agricultural pesticides on the environment.
Why are amphibians considered model organisms?
They also have permeable skin that can easily absorb toxic chemicals, making them a model organism for assessing the effects of environmental factors that may cause the declines of the amphibian population.

Summary
A bioindicator is any species (an indicator species) or group of species whose function, population, or status can reveal the qualitative status of the environment. The most common indicator species are animals. For example, copepods and other small water crustaceans that are present in many water bodies can be monitored for changes (biochemical, physiological, or behavioural) …
Overview
A bioindicator is an organism or biological response that reveals the presence of pollutants by the occurrence of typical symptoms or measurable responses and is, therefore, more qualitative. These organisms (or communities of organisms) can be used to deliver information on alterations in the environment or the quantity of environmental pollutants by changing in one of the following ways: physiologically, chemically or behaviourally. The information can be deduced through the st…
Plant and fungal indicators
The presence or absence of certain plant or other vegetative life in an ecosystem can provide important clues about the health of the environment: environmental preservation. There are several types of plant biomonitors, including mosses, lichens, tree bark, bark pockets, tree rings, and leaves. Fungi too may be useful as indicators.
Animal indicators and toxins
Changes in animal populations, whether increases or decreases, can indicate pollution. For example, if pollution causes depletion of a plant, animal species that depend on that plant will experience population decline. Conversely, overpopulation may be opportunistic growth of a species in response to loss of other species in an ecosystem. On the other hand, stress-induced sub-lethal eff…
Microbial indicators
Microorganisms can be used as indicators of aquatic or terrestrial ecosystem health. Found in large quantities, microorganisms are easier to sample than other organisms. Some microorganisms will produce new proteins, called stress proteins, when exposed to contaminants such as cadmium and benzene. These stress proteins can be used as an early warning system to detect changes in l…
Macroinvertebrates
Macroinvertebrates are useful and convenient indicators of the ecological health of water bodies and terrestrial ecosystems. They are almost always present, and are easy to sample and identify. This is largely due to the fact that most macro-invertebrates are visible to the naked eye, they typically have a short life-cycle (often the length of a single season) and are generally sedentary. Pre-exi…
See also
• Biological integrity
• Biological monitoring working party (a measurement procedure)
• Biosignature
• Ecological indicator
Further reading
• Caro, Tim (2010). Conservation by proxy: indicator, umbrella, keystone, flagship, and other surrogate species. Washington, DC: Island Press. ISBN 9781597261920.