What is a pipette?
A simple, critical, instrument in labs across the world, a pipette transports a measured volume of liquid safely and accurately. Pipettes can be as simple as plastic tubes and as complex as precise electronic devices.
Are dropper pipette dispensing systems made of glass or plastic?
Now that you have seen the applications for Dropper Pipette Dispensing Systems, we’ll share the pros and cons of the two material types – glass and plastic – to help you make your packaging decision. In the beginning, all pipettes were made of glass. However, over the years plastic pipettes have become increasingly popular.
What is a transfer pipette made of?
A transfer pipette Transfer pipettes, also known as Beral pipettes, are similar to Pasteur pipettes but are made from a single piece of plastic and their bulb can serve as the liquid-holding chamber.
What are the advantages of plastic materials in pipettes?
Some of the major advantages of plastic materials in pipettes include: Safety – Plastic is non-breakable and shows some degree of flexibility to help avoid any skin punctures. Potentially save you time and money - Traditional glass Pasteur pipette units can be damaged during shipping or handling. This breakage and waste adds to your product cost.
What are plastic pipettes used for?
Plastic pipettes such as polystyrene are basically disposable, so there is no cleaning time involved and pre-sterilized items are convenient in preventing contamination. Pipettes are generally used for moving small amounts of liquid or when measuring and dispensing liquid in mL units.
What are plastic pipettes made from?
low density polyethylene (LDPE)Practical one-piece plastic transfer pipettes (Pasteur pipettes), made from natural low density polyethylene (LDPE).
What are plastic pipettes called?
Pasteur pipettes are plastic or glass pipettes used to transfer small amounts of liquids, but are not graduated or calibrated for any particular volume. The bulb is separate from the pipette body. Pasteur pipettes are also called teat pipettes, droppers, eye droppers and chemical droppers.
How do you use a plastic pipette bulb?
0:221:38Using a Transfer Pipet - YouTubeYouTubeStart of suggested clipEnd of suggested clipThen slowly apply pressure on the bulb until the solution reaches the desired. Volume.MoreThen slowly apply pressure on the bulb until the solution reaches the desired. Volume.
What are the 3 types of pipettes?
What are the Different Types of Pipettes Used in Dentistry?Disposable Pipette. The disposable pipette is the most basic version of this tool. ... Graduated Pipette. ... Single-Channel Pipette. ... Multichannel Pipette. ... Repeat Dispensing Pipette.
What are the 2 different types of pipettes?
Serological pipettes are used in cell and tissue culture applications, and in general laboratory liquid dosage when more than 1mL volumes are pipetted. Serological pipettes are made of glass or polystyrene. Plastic, disposable pipettes are useful in applications where sterility is a requirement.
How accurate are plastic pipettes?
Graduated Pastettes enable measured transfer of liquids with +/- 10% accuracy.
What are the different kinds of pipette?
Eye dropperGraduated pipettePositive displacem... pipetteVan Slyke pipetteAir displacem... micropipett...Ostwald–F... pipettePipette/Types
What is the difference between pipette and dropper?
What is a graduated pipette vs a dropper? These two go hand-in-hand, for they are both interchangeable terms, with droppers allowing for controlled dispensing. With that said, a pipette, also called a pipet, pipettor, or chemical dropper is a laboratory instrument used to transport a measured volume of liquid.
How do you fill a plastic pipette?
0:170:55How To Use a Pipet Filler - YouTubeYouTubeStart of suggested clipEnd of suggested clipAnd e first insert the pipette in the pipette filler. Then press the a button to evacuate the air toMoreAnd e first insert the pipette in the pipette filler. Then press the a button to evacuate the air to draw the liquid up into the pipette. And E to empty the pipette.
What does a pipette measure?
A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz).
What is glass pipette?
Glass pipettes are laboratory tools used to transfer liquids between containers or to add reagents among other purposes. This class of pipettes works by creating a partial vacuum above the liquid-holding chamber, and selectively releasing this vacuum to draw up and dispense liquid.
What is a micropipette?
What is a micropipette? A micropipette is a common yet an essential laboratory instrument used to accurately and precisely transfer volumes of liquid in the microliter range. Micropipettes are available in single channel and multi channel variants.
What are transfer pipettes?
What are transfer pipets? Transfer pipets are disposable plastic pipets used to transfer small volumes of liquids. They consist of a long, slender tube and a molded bulb which can be gently compressed and relaxed to remove or dispense a desired liquid.
What is a pipette called?
Although specific descriptive names exist for each type of pipette, in practice any type of pipette will merely be referred to as a "pipette" and the desired device will be obvious from context. Sometimes, pipettes that dispense between 1 and 1000 μl are distinguished as micropipettes, while macropipettes dispense greater volumes.
What was the first pipette made of?
The first simple pipettes were made in glass, such as Pasteur pipettes. Large pipettes continue to be made in glass; others are made in squeezable plastic for situations where an exact volume is not required.
What is a pipetting syringe?
Pipetting syringes are hand-held devices that combine the functions of volumetric (bulb) pipettes, graduated pipettes, and burettes. They are calibrated to ISO volumetric A grade standards. A glass or plastic pipette tube is used with a thumb-operated piston and PTFE seal which slides within the pipette in a positive displacement operation. Such a device can be used on a wide variety of fluids (aqueous, viscous, and volatile fluids; hydrocarbons; essential oils; and mixtures) in volumes between 0.5 mL and 25 mL. This arrangement provides improvements in precision, handling safety, reliability, economy, and versatility. No disposable tips or pipetting aids are needed with the pipetting syringe.
What is a Pasteur pipette?
Pasteur pipettes are plastic or glass pipettes used to transfer small amounts of liquids, but are not graduated calibrated for any particular volume . The bulb is separate from the pipette body. Pasteur pipettes are also called teat pipettes, droppers, eye droppers and chemical droppers .
What is a volumetric pipette?
Typical volumes are 10, 25, and 50 mL. Volumetric pipettes are commonly used to make laboratory solutions from a base stock as well as prepare solutions for titration .
How to correct a pipette?
Corrective action: Use pipettes with large contoured or rounded plungers and buttons. This will disperse the pressure used to operate the pipette across the entire surface of the thumb or finger, reducing contact pressure to acceptable levels.
What are the safety regulations for pipettes?
The safety regulations included the statement: "Never pipette by mouth KCN, NH3, strong acids, bases and mercury salts". Some pipettes were manufactured with two bubbles between the mouth piece and the solution level line, to protect the chemist from accidental swallowing of the solution.
What is pipettes.com?
At Pipettes.com, we pride ourselves on the compliance-oriented calibration service we provide our clients, but part of our expertise involves having in-depth knowledge of all the instruments we service. This post will define the various types of pipettes and how to use them to ensure your tests provide the most precise results with the smallest margin of error:
How to use a pipette to measure volume?
To use this technique, press the plunger to first stop and slightly submerge the pipette tip into the liquid, aspirate your measured volume by releasing the plunger slowly to prevent bubbles. To dispense the liquid, place the tip against the side of the receptacle, then slowly press the plunger through the first stop to the final blow out position while ‘touching off’ the last drop from the tip.
How to remove liquid from a vessel?
Place the tip 45 degrees against the wall of the vessel receiving the liquid. Depress the plunger to the first stop, wait one second, press the plunger to the second stop and expel all the liquid while ‘touching off’ the last drop. Move the end of the tip away from the liquid and release the plunger to the rest position.
How to take a sample of liquid from a pipette?
Place the pipette tip against the wall of the receptacle and press the plunger to the first stop, holding the plunger in place and remove the tip from the receptacle. You will now have a sample of liquid that remains in the tip, but is not part of the measurement. You can then repeat the aspiration procedure and continue.
How to use a reverse pipette?
To use this technique, press plunger all the way through to the third stop position (all the way down), slightly submerge into liquid, and slowly release the plunger all the way back to the top and aspirate the liquid into the tip. Place the pipette tip against the wall of the receptacle and press the plunger to the first stop, holding the plunger in place and remove the tip from the receptacle. You will now have a sample of liquid that remains in the tip, but is not part of the measurement. You can then repeat the aspiration procedure and continue.
How many grades of pipette are there?
Within pipette calibration there are five widely used grades of pipettes, all of which have specific guidelines and requirements regarding use, testing, maintenance, and measurement. The five grades of pipettes include disposable/transfer, graduated/serological, single-channel, multichannel, and repeat pipette. From the most basic transfer pipette dropper to the advanced repeat dispensing pipettor, the manner in which the equipment is handled will impact the accuracy of the test results.
What is a single channel pipette?
A single-channel pipette is a non-disposable instrument, usually an air-displacement design which produces accurate measurement results with the use of one disposable tip. There are two common techniques associated with single-channel pipetting:
Why use plastic pipettes?
The main reasons are its unbreakable nature, cost, and compatibility with common laboratory chemicals.
What are pipettes made of?
In the beginning, all pipettes were made of glass. However, over the years plastic pipettes have become increasingly popular.
WHAT ARE DROPPER PIPETTES?
An eye dropper, also known as a Pasteur pipette, or dropper, is a device consisting of a small tube with a vacuum bulb that is used to transfer liquids one drop at a time. Back in the late 1800s there wasn’t a system to transport a solution without exposing it to the external environment, so Louis Pasteur invented the dropper pipette.
What are the two types of dropper pipette?
Now that you have seen the applications for Dropper Pipette Dispensing Systems, we’ll share the pros and cons of the two material types – glass and plastic – to help you make your packaging decision.
What are the components of a dropper pipette dispenser?
A Dropper Pipette Dispensing System has 3 key components – a bulb, cap, and pipette. We’ll focus here on the pipettes. Pipettes are available in glass or plastic and can be custom printed or marked with measurements, for accurate dosing.
Why is it important to choose the right dropper pipette dispenser?
When it comes to enhancing your customer’s experience, selecting the right Dropper Pipette Dispensing System is important. Packaging decisions for your oils, tinctures, serums and flavorings can make or break the user experience with your brand.
Why use pipettes?
In addition, wide user acceptance, ease of use, portability, and availability are common reasons to use pipettes across all markets. Included below are the primary drivers for pipette usage in selected markets.
Plastic Reduction Strategies
So how exactly have these companies committed to using less plastic? These methods include stackable pipette tip racks, refillable racks, and pipette tips sold in bags rather than new racks.
Choosing Recyclable Plastics
Most pipette tips and their boxes are made of either low density polyethylene (LDPE, plastic #4), or polypropylene (PP, plastic #5).
Consider Choosing Items That Use Less Material
Many laboratory supply companies offer products that are designed to use less plastic material in their construction or packaging than their competitors. This is usually done by creating thinner walls or streamlining the design in ways that attempt not to impact key performance indicators.
Overview
Specialized pipettes
Pipetting syringes are hand-held devices that combine the functions of volumetric (bulb) pipettes, graduated pipettes, and burettes. They are calibrated to ISO volumetric A grade standards. A glass or plastic pipette tube is used with a thumb-operated piston and PTFE seal which slides within the pipette in a positive displacement operation. Such a device can be used on a wide variety …
History
The first simple pipettes were made in glass, such as Pasteur pipettes. Large pipettes continue to be made in glass; others are made in squeezable plastic for situations where an exact volume is not required.
The first micropipette was patented in 1957 by Dr Heinrich Schnitger (Marburg, Germany). The founder of the company Eppendorf, Dr. Heinrich Netheler, inherited the rights and started the co…
Nomenclature
Although specific descriptive names exist for each type of pipette, in practice any type of pipette will merely be referred to as a "pipette" and the desired device will be obvious from context. Sometimes, pipettes that dispense between 1 and 1000 μl are distinguished as micropipettes, while macropipettes dispense greater volumes.
Common pipettes
Air displacement micropipettes are a type of adjustable micropipette that deliver a measured volume of liquid; depending on size, it could be between about 0.1 µl to 1,000 µl (1 ml). These pipettes require disposable tips that come in contact with the fluid. The four standard sizes of micropipettes correspond to four different disposable tip colors:
Calibration
Pipette recalibration is an important consideration in laboratories using these devices. It is the act of determining the accuracy of a measuring device by comparison with NIST traceable reference standards. Pipette calibration is essential to ensure that the instrument is working according to expectations and as per the defined regimes or work protocols. Pipette calibration is considered to be a complex affair because it includes many elements of calibration procedure and several c…
Posture and injuries
Proper pipetting posture is the most important element in establishing good ergonomic work practices. During repetitive tasks such as pipetting, maintaining body positions that provide a maximum of strength with the least amount of muscular stress is important to minimize the risk of injury. A number of common pipetting techniques have been identified as potentially hazardous due to biomechanical stress factors. Recommendations for corrective pipetting actions, made b…
Pipette stand
Typically the pipettes are vertically stored on holder called pipette stands. In case of electronic pipettes, such stand can recharge their batteries. The most advance pipette stand can directly control electronic pipettes.