What is Ife and PE serum? An IFE test detects immunoglobulins, also known as antibodies, in the blood, including IgA
Immunoglobulin A
Immunoglobulin A is an antibody that plays a crucial role in the immune function of mucous membranes. The amount of IgA produced in association with mucosal membranes is greater than all other types of antibody combined. In absolute terms, between three and five grams ar…
Immunoglobulin G
Immunoglobulin G is a type of antibody. Representing approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation. IgG molecules are created and released by plasma B cells. Each IgG has two antigen binding sites.
Immunoglobulin M
Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen. The spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production.
Monoclonal antibody
Monoclonal antibodies are antibodies that are made by identical immune cells that are all clones of a unique parent cell. Monoclonal antibodies can have monovalent affinity, in that they bind to the same epitope. In contrast, polyclonal antibodies bind to multiple epitopes and are usually made b…
Gel electrophoresis of proteins
Protein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium: SDS polyacrylamide gel electrophoresis (in short: gel …
Multiple Myeloma
Cancer of mature plasma cells in the bone marrow.
What is the IFE test used for?
Jan 24, 2022 · What Is Ife And Pe Serum? The IFE test detects immunoglobulins, also referred to as antibodies, within the bloodstream, including IgA, IgG, and IgM. The serum protein electrophoresis (SPEP) test measures specific proteins within the bloodstream to assist identify some illnesses. Proteins are substances comprised of smaller sized …
What is the difference between SPEP and Ife tests?
Nov 15, 2021 · Often asked: What is Ife and PE serum? November 15, 2021 Nora FAQ. The IFE test detects immunoglobulins, also known as antibodies, in the blood, including IgA, IgG, and IgM. The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to help identify some diseases.
Can a 24-hour urine Ife be added to the IFE test?
An immunofixation blood test, also known as protein electrophoresis, measures certain proteins in the blood. Proteins play many important roles, including providing energy for the body, rebuilding muscles, and supporting the immune system. There are two main types of proteins in the blood: albumin and globulin.
What does it mean to have an IEP serum test?
What is Ife and PE serum? An IFE test detects immunoglobulins, also known as antibodies, in the blood, including IgA, IgG, and IgM. It is most often ordered to evaluate a monoclonal globulin detected in a protein electrophoresis or to delineate a possible lymphoproliferative process, particularly myeloma. Click to see full answer.
What is Ife and PE serum test?
A serum immunofixation test measures specific proteins in your blood. It helps to identify certain illnesses that are otherwise hard to detect, such as multiple myeloma, a blood cancer. The test is also known as IFE, immunofixation electrophoresis, or protein electrophoresis.Nov 5, 2021
What is the blood test Ife?
What is an immunofixation (IFE) blood test? An immunofixation blood test, also known as protein electrophoresis, measures certain proteins in the blood. Proteins play many important roles, including providing energy for the body, rebuilding muscles, and supporting the immune system.Nov 15, 2021
What is urine IFE?
Urine immunofixation is a test that measures proteins in your urine. It specifically looks for monoclonal protein, or M-protein, which shouldn't be there in most cases. Doctors will be able to find out if you have conditions such as multiple myeloma or primary amyloidosis through urine immunofixation.Jun 3, 2021
Is Ife the same as Spep?
IFE is performed after the specimen is subjected to electrophoresis in the same process as SPEP but in multiple lanes.
How long is IFE?
The IFE test is used to identify monoclonal immunoglobulin gammopathies. It may also indicate conditions such as multiple myeloma or Waldenstrom's macroglobulinemia. Preparation: Fasting for 8-12 hours required. Test Results: 3-4 business days.
What is the most definitive test to confirm a diagnosis of multiple myeloma?
Bone x-rays X-rays can detect bone destruction caused by the myeloma cells. Often doctors will do a series of x-rays that includes most of the bones. This is called a bone survey or skeletal survey.Feb 28, 2018
What is normal for protein in urine?
Normal amount of protein in the urine are less than 150mg/day. High levels of protein in the urine are associated with rapid decline in kidney function. It affects about 6.7 percent of the United States population. It is seen more in elderly and people with other chronic illnesses.Jan 15, 2019
Is it good to have no monoclonal protein detected?
If no monoclonal protein is detected, further testing is not warranted. An expert panel of the College of American Pathologists developed the following guidelines for the laboratory evaluation of patients suspected of having one of these conditions (Arch Pathol Lab Med.
What causes increased gamma globulin?
Increased gamma globulin proteins may indicate: Blood cancers, including multiple myeloma, Waldenström macroglobulinemia, lymphomas, and chronic lymphocytic leukemias. Chronic inflammatory disease (for example, rheumatoid arthritis) Acute infection.
Is multiple myeloma curable?
Treatment for multiple myeloma can often help control symptoms and improve quality of life. But myeloma usually can't be cured, which means additional treatment is needed when the cancer comes back.
What is UPEP and SPEP?
Serum protein electrophoresis (SPEP) is used to determine the type of each protein present and may indicate a characteristic curve (ie, where the spike is observed). Urine protein electrophoresis (UPEP) is used to identify the presence of the Bence Jones protein in urine.
What is Ife in Yoruba?
The meaning of the word "ife" in Yoruba is "expansion"; "Ile-Ife" is therefore in reference to the myth of origin as "The Land of Expansion" (the word, Ile, as pronounced in modern Yoruba language, means house or home, which could make the name of the town mean "The House of Expansion").
Test Includes
Quantitation of serum immunoglobulins IgA, IgG, and IgM; IFE qualitative interpretation of heavy- and light-chain patterns; serum protein electrophoresis; quantitation of serum free κ and λ light chains plus κ:λ ratio
Expected Turnaround Time
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Collection
Sample should be allowed to clot and the serum separated as soon as possible to prevent hemolysis.
Methodology
Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains (IFE); protein electrophoresis on agarose media; turbidimetric quantitation of IgA, IgG, and IgM; turbidimetric quantitation of free κ and λ light chains
Additional Information
The NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines™) recommend the use of serum free light chain assays in the initial diagnostic work-up of multiple myeloma and related disorders.
Footnotes
1. NCCN Clinical Practice Guidelines in Oncology (NCCN Guidelines™) for Multiple Myeloma, Version 1.2011. Fort Washington, Pa: NCCN; 2011.
How is IFX performed?
The IFX test is performed on a blood sample. The blood sample is taken from your arm by a nurse or lab technician. The blood will be collected in a tube and sent to a lab for analysis. Your doctor will be able to explain your results.
What is IFX test?
The IFX test is often used to diagnose multiple myeloma or Waldenstrom’s macroglobulinemia, when symptoms of the disorders are present. Both conditions produce abnormal Ig. Clinical symptoms of multiple myeloma include: bone pain in the back or ribs. weakness and fatigue. weight loss. broken bones. recurrent infections.
What is the spike in immunofixation?
These are called monoclonal antibodies. In a serum immunofixation (IFX) test, they appear as a spike called an M spike. They are considered to be abnormal Ig.
What is an immunofixation serum test?
These proteins protect the body against disease. There are many different types of Ig. Certain diseases result in the growth of an excess number of antibody-producing cells.
Can needle sticks hurt after IFX?
People undergoing the IFX test may experience some discomfort when the blood sample is drawn. Needle sticks may result in pain or throbbing at the injection site during or after the test. Bruising may also occur. The risks of the IFX test are minimal.