What is peptone water used for in microbiology?
Peptone Water is used for the cultivation of non-fastidious microorganisms, indole testing, and as a basal medium for carbohydrate fermentation studies. Peptone Water is not intended for use in the diagnosis of disease or other conditions in humans. A general purpose growth medium that can be used as a base for carbohydrate fermentation studies.
What is the pH of peptone water?
Peptone water is formulated as per Shread, Donovan, and Lee. It is a broth medium used for the growth of the organism and a base for determining carbohydrate fermentation patterns of non-fastidious organisms. In addition, it is also used for the detection of indole production by the organism. Final pH (at 25°C) 7.2±0.2
What are the two components of peptone water?
The two basic components of peptone water are peptone and sodium chloride. Peptone provides nitrogenous and carbonaceous compounds, long-chain amino acids, vitamins provide essential nutrients. Sodium chloride provides the necessary electrolyte and maintains the osmotic balance of the medium.
What is Alkaline Peptone water used for?
1 Alkaline Peptone Water is commonly used for the enrichment of Vibrio cholera and Vibrio species from food, water and clinical samples. 2 The broth can also be used for direct microscopic examination of samples using the hanging drop method. 3 It is used as both enrichment and transport media. More items...
What is the difference between peptone and Peptone Water?
Peptone water is formulated as per Shread, Donovan and Lee (9). Peptone Water with pH adjusted to 8.4 is suitable for the cultivation and enrichment of Vibrio species. Peptone provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins provides essential nutrients.
Why is Peptone Water used in buffers?
Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods.
What is a peptone in microbiology?
Peptone is a protein derivative that is formed by the partial hydrolysis of proteins under acidic conditions. It is not coagulated by heat and is soluble in water. Peptone contains polypeptides and amino acids and it provides a readily available source of nitrogen and minerals for the growth of bacteria.
Is Peptone Water a diluent?
Peptone added to distilled water was tested as a diluent by Straka and Stokes (7) and proved to have advantages over distilled water or phosphate diluent for bacteria recovered from precooked frozen chicken and turkey pies.
What is the role of peptone in bacteriological media?
Peptone, Bacteriological is prepared by enzymatic digestion of selected fresh meat. Being highly nutritious it supports good growth of wide variety of microorganisms and can be used for identification of bacteria by performing various biochemical tests.
What is the pH of buffered Peptone Water?
7.2 ±Autoclave at 121°C for 15 min. Final pH 7.2 ± 0.2.
What is the Colour of peptone water?
Peptone Water may be modified for use in carbohydrate fermentation tests by the addition of Andrade's indicator. The indicator which is pink at pH 5.0 and yellow at pH 8.0 is prepared by adding sodium hydroxide to acid fuchsin until it becomes yellow. When added to Peptone Water it is colourless to slightly pink.
What is an example of a peptone?
Typical protein sources for peptones include meat, milk, soybean, yeast, and gelatin.
What is the difference between peptone water and nutrient broth?
Nutrient Agar vs Nutrient Broth Nutrient Broth is a liquid medium which is prepared to grow a wide variety of bacteria. Nutrient Agar contains peptone, yeast extract, beef extract, NaCl, agar, and water. Nutrient Broth contains peptone, yeast extract, beef extract, NaCl, and water.
How do you make Peptone Water buffer?
Peptone 10.0 g Sodium Chloride 5.0 g Disodium Phosphate (Na2HPO4) 7.0 g Monopotassium Phosphate (KH2PO4) 3.0 g Distilled water 1000 mL pH to 7.2±0.2 Page 2 Note: commercial Buffered Peptone Water (BPW; BD catalog number 218105) may be substituted for the above formulation.
How do you store Peptone Water?
Store the dehydrated medium at 24 °C and the prepared medium at 2-8 °C. Appearance Yellow colored, homogeneous, free flowing powder. Color and Clarity Yellow colored, clear solution without any precipitate. Cultural Response Cultural characteristics observed after 18-24 hours at 35-37 °C.
How long can you store Peptone Water?
According to ISO 6579-1:2017, prepared media can be stored up to 72 hours at 5°C. The evaluation confirms that prepared Readybag® Buffered Peptone Water could not only be stored for 72 hours at 5°C but also at room temperature.
Why is peptone used in media preparation?
Peptones are widely used as supplements for serum-free culture media. The growth-promoting activity of peptones may have a dual effect in batch cultures, while it may promote rapid cell growth in the first days of culture.
How do you make Peptone Water buffer?
Peptone 10.0 g Sodium Chloride 5.0 g Disodium Phosphate (Na2HPO4) 7.0 g Monopotassium Phosphate (KH2PO4) 3.0 g Distilled water 1000 mL pH to 7.2±0.2 Page 2 Note: commercial Buffered Peptone Water (BPW; BD catalog number 218105) may be substituted for the above formulation.
Why is peptone used in nutrient agar?
Peptones are the main source of organic nitrogen, especially via amino acids and long-chain peptides. Agar is used as a gelling agent. Nutrient agar can also be used for the cultivation of demanding microorganisms by enriching the medium with serum or blood.
What is composition of Peptone Water?
Peptone water is a microbial growth medium composed of peptic digest of animal tissue and sodium chloride. The pH of the medium is 7.2±0.2 at 25 °C and is rich in tryptophan. Peptone water is also a non-selective broth medium which can be used as a primary enrichment medium for the growth of bacteria.
What is peptone water?
Peptone water is the formulation of Shread, Donovan, and Lee. It is the simplest broth medium used for the growth of the organism and a base for determining carbohydrate fermentation patterns of non-fastidious micro-organisms. It is also used for the detection of indole production by the bacteria after the addition of Kovacs or Ehlrich reagent.
What is the difference between peptone and water?
Water is the source of hydrogen and oxygen. Sodium chloride is the source of electrolytes. Peptone is a complex mixture of partially digested proteins. It contains proteoses, amino acids, polypeptides, phosphates, minerals (K, Mg), and accessory growth factors like nicotinic acid and riboflavin. Peptone used in this medium is rich in tryptophan content. The presence of indole can be detected using either Kovacs or Ehlrich reagent. Peptone Water is also applied as a base for carbohydrate fermentation studies with the addition of sugar and indicators such as bromothymol blue, bromocresol purple, or phenol red. Gas formation is achieved using Durham’s tube.
Anti-Bacteriological Effect of Nanoscaled Inorganic Particles in Coating Matrices
For most of the applications mentioned, a coating is needed which shows long-term biocide effectiveness. The biocide nanoparticles are especially surface modified for the incorporation into different matrices and especially in paint systems. Figure 6.9 shows a typical example of such a coating on a metal substrate.
Biological NMR Part B
Whitney N. Costello, ... Kendra K. Frederick, in Methods in Enzymology, 2019
Engineering Fundamentals of Biotechnology
S. Sood, ... A. Kumar, in Comprehensive Biotechnology (Second Edition), 2011
Microbiology of Atypical Environments
Indrani Karunasagar, ... Ballamoole Krishna Kumar, in Methods in Microbiology, 2018
Environmental Biotechnology and Safety
A diverse group of peptones obtained by enzymatic hydrolysis of wastewater from the industrial processing of octopus showed their effectiveness to promote the growth of lactic acid bacteria (LAB) and the production of bacteriocins. The highest nisin formation by L.
What is alkaline peptone water?
Alkaline Peptone Water is a broth medium for the enrichment of Vibrio species from food, water and clinical samples.
What happens to contaminating organisms incubated for a long time?
Prolonged incubation will result in growth of the suppressed contaminating organisms to develop.
