Gram Stain Reagents
- Primary Stain (Crystal Violet). It is an intensely purple-colored organic compound chemically called triphenylmethane...
- Mordant (Gram’s Iodine). It is an aqueous solution of iodine and potassium iodide used as mordant in Gram staining.
- Decolorizing Solution. It is either acetone or ethanol (95%) or a mixture of acetone and ethanol in ratio...
What reagents could be used in Gram stain?
- Carbolfuchsin.
- acid-alcohol.
- Methylene blue.
What is the most important reagent in Gram staining?
What is the most important reagent in the Gram stain method? The primary stain of the Gram’s method is crystal violet. Crystal violet is sometimes substituted with methylene blue, which is equally effective. The microorganisms that retain the crystal violet-iodine complex appear purple brown under microscopic examination.
What is the Order of reagents used in the Gram stain?
Explanation: Gram staining is a type of differential staining. In this process the fixed bacterial smear is subjected to the following staining reagents in the order listed: crystal violet, iodine solution, alcohol (decolorizing agent), and safranin.
Which bacteria will not stain using the Gram staining method?
This waxy barrier also prevents stains from penetrating the cell, which is why the Gram stain does not work with mycobacteria such as Mycobacterium, which are pathogens of humans and animals. For these bacteria, the acid–fast staining technique is used. Figure 6. Acid-fast bacilli in sputum
What color is a Gram negative?
The destained gram negative bacteria will stain a pinkish red
When do we use acid fast stain?
We use acid fast stains when the organism
What is the mordant in chemistry?
The mordant, which is the Gram's Iodine
Why do bacteria turn pink?
All bacteria will appear pink because there was not an insoluble complex added after the first step which is crystal violet