Full Answer
What is the optimal temperature of your enzyme?
This optimal temperature is usually around human body temperature (37.5 oC) for the enzymes in human cells. Above this temperature the enzyme structure begins to break down (denature) since at higher temperatures intra- and intermolecular bonds are broken as the enzyme molecules gain even more kinetic energy.
How do you find the optimal temperature of lactase?
The reaction rate increases from 35 degrees F up to the range of 125 to 135 degrees F where it drops off dramatically. So by definition, 125 to 135 degrees F is the "optimum temperature" (range) for lactase activity.
What is the optimal temperature for lactase and why?
The optimum temperature for lactase is about 48 °C (118.4 °F) for its activity and has an optimum pH of 6.5.
What are the optimal temperatures and pH of lactase?
E. coli lactase is readily inactivated at pH 5.0, and its optimal activity at 36°C. is reached between pH 7.0 and pH 7.5. The optimal temperature for its action was found to be 46°C. when determinations were carried out after an incubation period of 30 minutes.
What is the optimal temperature?
The optimum temperature range for humans depends on various factors, but in general the recommended range is 65°F to 70°F.
Why your results of the optimal temperature of the lactase enzyme make sense for the human enzyme?
The optimal temperature makes sense for human enzymes because human body temperature is just below $)C. At the optimal temperature or the average human body temperature the structure of a protein is not denatured.
What is the optimal pH for lactase enzyme activity?
The optimum pH for lactose hydrolysis is 6.5, which is close to the pH of milk. Heating lactase for 1 min results in 97% inactivation at 60 C and complete inactivation at 70 C. Some inactivation occurs at 40 C over 1-h incubation.
What is the optimal pH for lactase enzyme activity quizlet?
Lactase has optimal activity at pH ~ 7.