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Composition of Thioglycollate Broth.
What is thioglycollate medium used for?
Fluid Thioglycollate Medium. Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria. Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar.
What is the composition of thioglycollate?
Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria. Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar. The sodium thioglycollate, thioglycollic acid, and L-cystine reduce the oxygen to ...
What is thioglycollate broth?
Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria. Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar.
What is the function of sodium thioglycollate?
The sodium thioglycollate, thioglycollic acid, and L-cystine reduce the oxygen to water. Methylene blue is an indicator that is colorless in an anaerobic environment and greenish-blue in the presence of oxygen.
What does thioglycollate test for?
Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria. Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar.
What is the purpose of the fluid thioglycollate broth as a biochemical test?
Thioglycollate broth is used to find out the growth characteristics of various bacteria based on their oxygen requirements.
What is the indicator in fluid thioglycollate and what is its purpose?
In Fluid Thioglycollate Medium with indicator, resazurin is an oxidation-reduction indicator, being pink when oxidized (as oxygen is absorbed into the medium) and colorless when reduced.
What is the main purpose of adding sodium thioglycollate and cysteine in fluid thioglycolate broth?
The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.
What is the purpose of using thioglycollate as growth medium in assessing oxygen requirements of microbes?
Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. It acts as an enrichment broth which is most frequently used in diagnostic bacteriology....Composition of Thioglycollate Broth.IngredientsGms/literSodium thioglycollate0.55 more rows•Jan 7, 2022
What is the purpose of adding resazurin indicator in fluid thioglycollate broth?
Resazurin is an oxidation-reduction indicator that turns pink when increased oxidation has occurred, it is colorless when reduced. Agar: The addition of a small amount of agar in Thioglycollate medium aids in the initiation and growth of small inocula and anaerobes by impeding the diffusion of oxygen into the medium.
Why can media such as thioglycollate be used for cultivation of anaerobes?
Why can media such as brain heart infusion and thioglycollate be used for the cultivation of anaerobes? Anaerobes cannot be cultivated in the presence of atmospheric oxygen.
What is the principle of thioglycollate?
Principle of Thioglycollate Broth. Dextrose, pancreatic digest of casein, yeast extract, L-cystine provide the growth factors necessary for bacterial multiplication. L-cystine and sodium thioglycollate allows Clostridium and other strict anaerobes to grow in this medium even under aerobic conditions. Sodium thioglycollate act as a reducing agent ...
What is thioglycollate medium used for?
When anaerobic infection is suspected, thioglycollate medium is recommended to isolate strict anaerobes from the blood.
What is thioglycolate broth?
References. Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. It acts as an enrichment broth which is most frequently used in diagnostic bacteriology.
Why should thyoglycollate media not be re-heated more than once?
Thioglycollate media should not be re-heated more than once because toxic oxygen radicles are formed on reheating.