What is pGLO plasmid used for?
The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably for the green fluorescent protein (GFP) and the ampicillin resistance gene.
What are the reporter genes in plasmid plasmids?
The plasmid contains several reporter genes, most notably for the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from the jelly fish Aequorea victoria.
What is the role of marker genes in plasmid selection?
Complete answer: -Selectable marker genes are found in plasmids and they help in the selection of the transformants from the non-transformants. … -Selectable marker genes may give resistance to antibiotics especially the ampicillin-resistant and the tetracycline-resistant sites which are seen in the plasmid pBR322.
How can I induce GFP in bacteria containing the pGLO plasmid?
GFP can be induced in bacteria containing the pGLO plasmid by growing them on +arabinose plates. pGLO is made up of three genes that are joined together using recombinant DNA technology. They are as follows:
What genes does the pGLO plasmid carry?
The pGLO plasmid contains both the promoter (pBAD) and araC gene, but araB, araA, and araD have been replaced by the single gene that codes for GFP, which serves as a reporter gene.
What is the function of the bla gene in the pGLO plasmid?
The pGLO plasmid contains a gene (bla or ampR) for a protein called β-lactamase, which hydrolyzes antibiotics that have a β-lactam ring and makes host cells resistant to compounds like ampicillin.
Does pGLO plasmid contain GFP?
The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene.
What is the pGLO gene?
Other pGLO features bla — also known as the ampicillin resistance gene; a gene that encodes the enzyme β-lactamase, which breaks down the antibiotic ampicillin. The bla gene, like all genes, includes a promoter and a coding region.
What does the GFP gene do?
Biologists use GFP as a marker protein. GFP can attach to and mark another protein with fluorescence, enabling scientists to see the presence of the particular protein in an organic structure. Gfp refers to the gene that produces green fluorescent protein.
What two pieces of genetic information does pGLO plasmid code for?
1) The pGLO plasmid contains the gene for GFP and a gene for resistance to the antibiotic ampicillin.
Where does the GFP gene come from?
Green fluorescent protein (GFP) was originally derived from the jellyfish Aequorea victoria (Prendergast and Mann, 1978). It has 238 amino acid residues and a green fluorophore, which is comprised of only three amino acids: Ser65-Tyr66-Gly67.
What are the four main parts of the pGLO plasmid?
The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, which is a protein that regulates expression from the arabinose BAD promoter (PBAD).
Which pGLO gene has the genetic information to make AMP resistant cells glow?
Meaning, the plasmid DNA, or pGLO, contains a gene for Green Fluorescent Protein (GFP) as well as the gene for resistance to the antibiotic ampicillin. The GFP comes from the "Aequorea victoria" jellyfish. The protein causes the jellyfish to fluoresce and glow in the dark.
What is pGLO quizlet?
What is pGLO? a type of genetically engineered plasmid that encodes the gene for GFP and a gene for resistance to the antibiotic ampicillin.
Which gene is responsible for turning on the glow?
The cells that glow turn on the green fluorescent protein (GFP) gene naturally found in jellyfish DNA. The non-glowing cells keep this gene off.
What is the purpose of the pGLO plasmid in the transformation experiment?
The pGLO System With the pGLO transformation kit, students use a simple procedure to transform bacteria with a gene that codes for Green Fluorescent Protein (GFP). The real-life source of this gene is the bioluminescent jellyfish Aequorea victoria, and GFP causes the jellyfish to fluoresce and glow in the dark.
How to purify fluorescent protein from pglo?
Use chromatography to purify glowing green fluorescent protein from your pGLO bacteria.
What is the origin of pGLO?
ori — origin of pGLO plasmid DNA replication (essential for making more copies of the plasmid) bla — gene that encodes β-lactamase, an enzyme that breaks down the antibiotic ampicillin; transformants expressing the bla gene can be selected by placing ampicillin in the growth medium.
How to select bacteria containing the bla gene?
Bacteria containing the <em>bla</em> gene can be selected by placing ampicillin in the growth medium .</p> <p><strong>ori</strong> — the origin of pGLO plasmid DNA replication</p> <p><em><strong>araC</strong></em> — a gene that encodes the regulatory protein that binds to the pBAD promoter.
What is pglo transformation?
With pGLO bacterial transformation, students learn about genetic engineering as they transform a non-virulent laboratory strain of Escherichia coli ( E. coli) with the pGLO plasmid. The procedure involves the CaCl 2 /heat shock method, which is a standard technique used in many research and biomanufacturing laboratories.
What is the GFP gene used for?
First isolated from the marine jellyfish Aequorea victoria, the gene encoding GFP is used in cellular and molecular biology as a reporter to detect gene expression in transgenic organisms. Bio-Rad Explorer pGLO Plasmid and GFP Kits use the pGLO plasmid, which contains the GFP gene, to enable hands-on learning about the central dogma, ...
Where does AraC bind to DNA?
The AraC protein binds to the DNA at the binding site for RNA polymerase (the beginning of the arabinose operon).</p> <p>When arabinose is present in the environment, bacteria take it up. Once inside, the arabinose interacts directly with AraC, which is bound to the DNA.
Which promoter binds AraC-arabinose and promotes RNA polymerase binding and transcription?
pBAD promoter — binds AraC-arabinose and promotes RNA polymerase binding and transcription of the GFP gene
What is a marker gene?
A marker gene is one that produces an identifiable phenotype, allowing us to easily recognize the cells that have taken up a recombinant plasmid. What marker gene is carried by the pGLO plasmid?
What is a vector in biology?
The vector is often a small, circular piece of DNA that can exist outside of the bacterial chromosome called a .
