How to determine total fat content?
What are the key parameters used to determine food quality?
What determines the fat content in raw materials?
Why is fat content determined in water and sewage?
Total Fat Analysis in Milk using the Gerber Method
The Gerber Method is a primary and historic chemical test to determine the fat content of milk and other substances. The Gerber Method is the primary testing method in Europe and much of the world. The fairly similar Babcock test is used primarily in the United States, although the Gerber Method also enjoys significant use in the U.S. as well.
Total fat determination in meat products according to Weibull-Stoldt ...
A simple and reliable procedure for fat determination of meat products according to Weibull-Stoldt is introduced. The sample is hydrolyzed with the HydrolEx H-506.
Approach to fat analysis of foods - ScienceDirect
Behind the nutritional labelling of food fat hides a problem of definition of fat, as well as an analytical problem. Although triacylglycerols are the prevailing structure of the food lipids in most cases, there are exceptions, too.
Improved methods for analysis of fats and fatty acids ... - AAFCO
Reaction conditions for one-step extraction/methylation of feedstuffs Dry sample containing 10-50 mg fatty acid in a 20mm x 150mm tube withteflonlined cap Add organic solvent containing 4 mg internal standard Add 3 ml 5% acetyl-Cl in methanol or 2% methanolic sulfuric acid Incubate at 70 -90C for 2 hr, or at 50C overnight (preferred) Cool, add 1 ml hexane
Abstract
Determination of food lipid content is important because of regulatory requirements, nutritive value, and functional properties. This chapter includes the typical required sample preparation steps of predrying the sample, particle size reduction, and acid hydrolysis.
Notes
The author of this chapter wishes to acknowledge Dr. David Min (deceased) who was the key author on this Fat Analysis chapter for the first to fourth editions of this textbook.
Copyright information
Open Access This chapter is licensed under the terms of the Creative Commons Attribution-NonCommercial 2.5 International License (http://creativecommons.org/licenses/by-nc/2.5/), which permits any noncommercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author (s) and the source, provide a link to the Creative Commons license and indicate if changes were made..
What is fat soluble vitamin?
For nutrition labeling purposes, fat is defined as the sum of the fatty acids in the food, regardless of source, and this is expressed as triglyceride equivalents. These fatty acids may be present as the following: Table 2: Fatty Acids. Free fatty acids.
How to do fat by GC?
FAT by GC (FATGC) The following steps are taken in the FATGC procedure: Step 1: Triglycerides and other molecules containing fatty acids are released from food matrices using an acid or base hydrolysis. Step 2: The triglycerides and molecules are extracted into a mixture of ethyl and petroleum ether.
What are fats and lipids?
Fats are primarily the triesters of fatty acids and glycerol and thus are commonly called triglycerides. Solid triglycerides are referred to as fat while liquid triglycerides are called oils. Lipids, on the other hand, include all the “fatty” materials—the substances dissolved in a fat-solubilizing solvent in a food.
How does acid hydrolysis work?
Acid Hydrolysis. The acid-hydrolysis method extracts fat from the sample by subjecting it to hydrochloric acid and mixed ethers. The following steps are taken in the acid hydrolysis procedure: Step 1: The hydrochloric acid breaks fatty acids from the glycerides, glycolipids, phospholipids, and sterol esters.
Why is the solvent extraction method used?
The solvent extraction-gravimetric method can be used for quick fat determinations and relative comparisons between samples. However, it’s important to note that the results can be approximately 10% higher than fat revealed in results from the gas chromatograph (GC) method. These erroneously high results are due to certain nonfat components that are typically extracted with the fat. These nonfat components include:
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What is the purpose of a gas chromatograph?
The gas chromatograph (GC) method is used for accurate nutritional labeling to include trans fats, and it’s used for research projects. The GC method also provides accurate quantitation of total fat, as well as the individual fatty acids, which include saturated fat, monounsaturated fat, polyunsaturated fat, and trans fatty acids.
How to determine total fat content?
For foodstuffs, the most commonly used method for determining the total fat content is extraction using acid digestion, i.e. the Weibull-Stoldt/Weibull-Berntrop method. The sample is heated up with hydrochloric acid in order to digest proteins and release the bound lipids.
What are the key parameters used to determine food quality?
Together with water, protein, fibre and sodium content, fat content is one of the five key parameters used in assessing food quality. Fat content is also significant in assessment of animal feed, and is determined as crude fat within the framework of Weende analysis. In addition to other parameters, fat content is often a decisive factor ...
What determines the fat content in raw materials?
The processing industry determines the fat content in raw materials to assess their suitability for specific processing steps and analyses the fat content of finished products as part of quality control.
Why is fat content determined in water and sewage?
In the environmental area, the fat content in water and sewage is determined in order to assess the body of water.