Why Safranin is used for staining plant material in the experiment? The safranin is used in the agreement because the safranin is used to peel the stomata and the glycerine which is used in that experiment which helps to keep the stomata wet so that the stomata can be easily visible and the particles of the stomata can be very much visible for the experiment.
Why is safranin used to stain plant cells instead of methylene blue?
The safranin stain is a cheaper and safer-lab stain, and also provide a strong contrast to the crystal violet, which allows easy and rapid detection of the plant's vascular tissue. Whereas, methylene blue would not provide a strong contrast to the crystal violet as the safranin does. That’s why safranin is convenient to stain plant cell.
Why is safranin used in stomata extraction?
The safranin is used in the agreement because the safranin is used to peel the stomata and the glycerine which is used in that experiment which helps to keep the stomata wet so that the stomata can be easily visible and the particles of the stomata can be very much visible for the experiment. Click to see full answer.
What is safranin stain made of?
It stains lignified, suberized, cutinized and chitinized structures as also nucleoli, chromosomes and centrosomes. Various formulae are available for making safranin solution; but generally a 1% solution in 50% alcohol is good enough for most plant materials.
What is the function of safranin in cell culture media?
Safranin is a dye which can be taken up by a cell and it gives a pink colour. The cell as well as the background is transparent and it is difficult to visualise the cells as such. Staining imparts colour the cell or its components and enhances its contrast and makes it easier to see the structure of the cells.
Why safranin is used to stain plant cells?
Why is safranin convenient to stain plant cells whereas a methylene blue solution is convenient for animal cells? The safranin stain is a cheaper and safer-lab stain, and also provide a strong contrast to the crystal violet, which allows easy and rapid detection of the plant's vascular tissue.
What is the purpose of using safranin?
Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red. This is the classic counterstain in both Gram stains and endospore staining. It can also be used for the detection of cartilage, mucin and mast cell granules.
Why safranin is used to stain stomata?
Dear student, Safranin is used to stain leaf epidermis as safranine is a staining dye, it imparts red colour to cells and tissue thereby highlighting them when seen under microscope.
Which stain is used for plant cell?
Safranin is a staining agent used in histology and cytology to color the cells and visualize them under the microscope.
How does safranin work in Gram staining?
A counterstain, such as the weakly water soluble safranin, is added to the sample, staining it red. Since the safranin is lighter than crystal violet, it does not disrupt the purple coloration in Gram positive cells. However, the decolorized Gram negative cells are stained red.
Why is safranin used as a counterstain in endospore staining?
The safranin counterstain is used on the slide to give color to the vegetative cells. The endospores will have retained the malachite green, appearing green (sometimes a little bluish), and the vegetative cells will be brownish-red or pinkish.
Why do we use safranin as a stain Class 9?
safronnin is a basic stain.it is light than other stains such as methylene blue, crystal violet etc,... safronnin can be absorbed easily into the tissues or cells. bascically we use it in plant cell staining and micro staining.
Why do we use safranin solution while preparing a temporary slide in the laboratory?
Safranin is used to peel off the upper layer of stomata and also to dye the layer purple to see it clearly under the microscope. Glycerine is used to keep the stomata moisturized and wet so that the particle of stomata are easily visible.
What is safranin O staining?
General description. Safranin-O, also known as basic red 2, is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring all cell nuclei red. It can also be used for the detection of cartilage, mucin and mast cell granules.
Why do we stain plant cells?
Why Stain Cells? The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
Why is methylene blue used to stain plant cells?
Methylene Blue is a cationic stain and it is used to stain the nucleus because it has a positive ionic charge, it will interact with tissue cell nuclei that have a negative ionic charge.
What is plant stain?
The vascular tissues of plants can be distinguished by their structure and molecular composition using stains that react differentially with cellulose and lignin. In the microscopic analysis of animal and plant tissues, thin sections of tissue are required to achieve a slice with only one or a few layers of cells.
Why is methylene blue used in plant cells?
But if you are talking in general methylene blue is used because it stains animal nucleus better than other stains do. Same is the reason for using safrannin to stain plant cells. Methylene blue can stain dead cells too. Thanks for A2A.
Is methylene blue a basic dye?
At first you have to know that Methylene Blue is basic dye. As we know that the Cell wall and cell membrane both are acidic in nature…. So when we use dye on smear, World's fastest chemical reaction —”acid-base reaction” occurs, as acid and base attracts each other, so dye binds to cellular structures. ^^structure of Methylene Blue^^.
Can safrannin stain dead cells?
Same is the reason for using safrannin to stain plant cells. Methylene blue can stain dead cells too. Thanks for A2A. Malachite green - a blue-green counterstain to safranin in Gimenezstaining for bacteria. This stain can also be used to stain spores.Methylene blue - stains animal cells to make nuclei more visible.
What is safranin stain?
Safranin is a basic stain that easily stains nucleic acids inside a cell. As with many stains, the choice of substrate matters a lot. For example, when staining root tip cells, the roots should not be too old (meaning, avoid taking the tips of roots that are too long).
What is the best stain for root tip cells?
However, the cost of these stains is quite high. Personally, I find safranin, which is another stain, more flexible and more cost effective than either of these stain—especially for staining root tip cells for chromosome ...
What is safranine used for?
Safranine is an azo dye commonly used for plant microscopy , especially as a stain for lignified tissues such as xylem. Safranine fluorescently labels the wood cell wall, producing green/yellow fluorescence in the secondary cell wall and red/orange fluorescence in the middle lamella (ML) region. We examined the fluorescence behavior ...
What is the role of iron reducing bacteria in soil?
Iron-reducing bacteria (IRB) are crucial for electron transfer in anaerobic soil microsites. The utilization of the energy gathered by this mechanism by decomposers of organic matter is a challenging and fascinating issue. We hypothesized that bacteria reducing Fe (III) (oxyhydr)oxides to soluble Fe (II) obtain electrons from reduced soil organic matter (SOMr) involving lignin oxidation. Iron-reducing bacteria were isolated from topsoils of various climates (humid temperate, cold temperate, subpolar), vegetation types (mostly grasslands and forests), and derived from various parent materials treatments assigned as Granitic, Volcanic-allophanic, Fluvio-glacial, Basaltic-Antarctic and Metamorphic. After the screening of IRB by phospholipid fatty acid (PLFA) analysis and PCR identification (full-length 16S rDNA), the IRB were inoculated to 20 samples (five soils and 4 replicates) and a broad range of parallel processes were traced. Geobacter metallireducens and Geobacter lovleyi were the main Geobacteraceae-strains present in all soils and strongly increased the activity of ligninolytic enzymes: lignin peroxidase and manganese peroxidase. Carbon dioxide (CO2) released from IRB-inoculated soils was 140% higher than that produced by Fenton reactions (induced by H2O2 and Fe (II) addition) but 40% lower than in non-sterile soils. CO2 release was closely correlated with the produced Fe (II) and H2O2 consumption. The highest CO2 was released from Basaltic-Antarctic soils with the highest Fe content and was closely correlated with lignin depolymerization (detection by fluorescence images). All IRB oxidized the lignin contained in the SOM within a wide pH range and in soils from all parent materials. We present a conceptual model showing electron shuttling from SOM containing lignin (as a C and energy source) to IRB to produce energy and promote Fe (III) (oxyhydr)oxides reduction was proposed and discussed.
How does altitude affect saplings?
The altitude is an important factor to affect the growth and development of saplings of the tree. However, the effect of altitude on the growth and properties of wood during their young stage it has been little studied. This study, therefore, aimed to evaluate the influence of two different altitude steps: 795 m (a.s.l. low-altitude) and 1350 m (a.s.l. high altitude) on the morphological, anatomical and wood density properties of saplings of Abies nordmanniana subsp. equi-trojani [Asch. & Sint. ex Boiss] Coode & Cullen (Trojan fir). Trojan fir is an endemic species in Turkey and its morphology and anatomy have less studied in the literature. The functional traits and wood density properties differed significantly between the two altitudes. The saplings grown at low-altitude showed greater taper degree, pith radius, pith proportion, and bark proportion than high-altitude. However, stem height, stem diameter, node number, and xylem proportion were found to be higher in saplings grown at high-altitude than low-altitude. Wood cell anatomy also varied significantly between two altitudes such that ring width, ray numbers, tracheid length, and tracheid width were higher at low-altitude, whereas ray height, ray width, tracheid lumen width, and tracheid wall thickness were greater at high-altitude. This study, therefore, suggested that the growth and development of fir saplings were better when they were grown at high-altitude than low-altitude.
What is Commelina benghalensis used for?
is used as a traditional medicine in treating numerous ailments and diseases such as infertility in women, conjunctivitis, gonorrhea, and jaundice. This study used light and electron microscopy coupled with histochemistry to investigate the micromorphology, ultrastructure and histochemical properties of C. benghalensis leaves and stems. Stereo and scanning electron microscopy revealed dense non-glandular trichomes on the leaves and stems and trichome density was greater in emergent leaves than in the young and mature. Three morphologically different non-glandular trichomes were observed including simple multicellular, simple bicellular and simple multicellular hooked. The simple bicellular trichomes were less common than the multicellular and hooked. Transmission electron micrographs showed mitochondria, vesicles and vacuoles in the trichome. The leaf section contained chloroplasts with plastoglobuli and starch grains. Histochemical analysis revealed various pharmacologically important compounds such as phenols, alkaloids, proteins and polysaccharides. The micromorphological and ultrastructural investigations suggest that Commelina benghalensis L. is an economically important medicinal plant due to bioactive compounds present in the leaves and stems.
How does seasonality affect trees?
Climate seasonality is one of the important environmental variables that can drive changes in many properties of trees. However, how different tree species regulate their growth and development during changes of the growing period particularly at sapling stage is little known. This study, therefore, evaluated how morphological, anatomical, and soil properties differed in two growing season periods (March vs. September) between oak and black locust saplings across one growing season. Wood cell anatomical properties and soil characteristics were considerably influenced by seasonality. Each species showed greater wood cell anatomical characteristics in March than in September. Soil physical and chemical properties also showed great variance between March and September: soil pH, amount of Mg and Al concentrations in soil were higher in March while organic matter and total N concentration in soil showed greater values in September. Growth and development performance were also compared between two species. We found fibre cell characteristics, stem height, stem diameter and node number were to be greater in oak than in black locust for both early- and late growing periods. The results suggested that oak showed better adaptation to their local environments than black locust.