
How to take an arterial blood gas sample?
- Approach the patient, introduce yourself and ask the patient to state their full name.
- Place the patient on their back, lying flat. ...
- Locate the radial artery by performing an Allen test (see Annex I) for collateral circulation. ...
- Perform hand hygiene, clear off a bedside work area and prepare supplies. ...
How to draw blood from an arterial line?
- Insert one of the DISCARD tubes into the vacutainer. Be careful to use tubes that will not be confused with actual blood samples.
- Depress the blood tube to activate the vacuum.
- Open up the flow of blood by turning the stopcock 90 degrees toward the flush system. This is open between the patient and sampling port.
- Allow a minimum of 3 mL discard to be drawn from the arterial line.
- Quickly turn the stopcock to 45 degrees ( half-way between the patient and sampling port) when sufficient blood volume has been collected to avoid excess blood removal.
- Remove the tube to release the vacuum and discard into the sharps container.
What would an arterial blood gas test reveal?
An arterial blood gases (ABG) test is a blood test that measures the acidity, or pH, and the levels of oxygen (O2) and carbon dioxide (CO2) from an artery. The test is used to check the function of the patient’s lungs and how well they are able to move oxygen into the blood and remove carbon dioxide.
Who can draw arterial blood?
You are likely to have an arterial blood gas test in a hospital, but your doctor may be able to do it in their office. Your doctor or another health care worker will use a small needle to take some of your blood, usually from your wrist. Sometimes they take it from an artery in your groin or on the inside of your arm above your elbow.

How do you take an arterial blood sample?
For an arterial blood gas test, a respiratory therapist will take a sample of blood from one of your arteries. This is because there are higher oxygen levels in blood from an artery than blood from a vein. A respiratory therapist usually takes the sample from an artery inside your wrist known as the radial artery.
What does an arterial blood test show?
An arterial blood gases (ABG) test is a blood test. It measures the acid-base balance (pH) and the levels of oxygen and carbon dioxide in the blood. It uses blood drawn from an artery. This is where the levels of oxygen and carbon dioxide can be measured before they enter body tissues.
Why do they do arterial blood draws?
An arterial stick is done to get blood samples from arteries. Blood samples are mainly taken to measure gases in the arteries. Abnormal results may point to breathing problems or problems with the body's metabolism . Sometimes arterial sticks are done to get blood culture or blood chemistry samples.
What is the difference between arterial blood and venous blood?
The main difference between arterial and venous blood is that arterial blood is oxygenated whereas venous blood is deoxygenated. The arterial blood is bright red in color and the venous blood is blackish red in color.
What level of oxygen is dangerously low?
If you're using an oximeter at home and your oxygen saturation level is 92% or lower, call your healthcare provider. If it's at 88% or lower, get to the nearest emergency room as soon as possible.
What are the disadvantages of arterial blood collection?
Collection of arterial blood has its disadvantages. It is more difficult to obtain than venous or capillary blood sample, it is more painful for the patient and it can be potentially dangerous, if the correct procedure is not followed (1).
Why is blood taken from veins and not arteries?
Veins are favored over arteries because they have thinner walls, and thus they are easier to pierce. There is also lower blood pressure in veins so that bleeding can be stopped more quickly and easily than with arterial puncture.
What color is arterial blood?
bright redSince blood in arteries is usually full of oxygen, the hemoglobin in the red blood cells is oxygenated. The resultant form of hemoglobin (oxyhemoglobin) is what makes arterial blood look bright red.
What are normal arterial blood gas values?
According to the National Institute of Health, typical normal values are: pH: 7.35-7.45. Partial pressure of oxygen (PaO2): 75 to 100 mmHg. Partial pressure of carbon dioxide (PaCO2): 35-45 mmHg.
What labs can be drawn from arterial line?
(RT and/ or RN are capable of drawing a blood gas from an arterial line.) Arterial blood gas values may be indicated before and after the start or discontinuation of oxygen administration to the patient, after ventilator changes, and when a change in patient status has been noted.
How painful is an arterial blood gas test?
You may have a few minutes of discomfort during or after the test. Collecting blood from an artery typically hurts more than drawing it from a vein. Arteries are deeper than veins, and there are sensitive nerves nearby. You also may feel lightheaded, faint, dizzy, or nauseated while your blood is drawn.
Why would a physician order an ABG?
The blood gas test can determine how well your lungs are able to move oxygen into the blood and remove carbon dioxide from the blood. Imbalances in the oxygen, carbon dioxide, and pH levels of your blood can indicate the presence of certain medical conditions. These may include: kidney failure.
What is arterial blood sampling?
An arterial blood sample is collected from an artery, primarily to determine arterial blood gases. Arterial blood sampling should only be performed by health workers for whom the procedure is in the legal scope of practice for their position in their country and who have demonstrated proficiency after formal training.
Which artery is used for blood collection?
Several different arteries can be used for blood collection. The first choice is the radial artery, which is located on the thumb side of the wrist; because of its small size, use of this artery requires extensive skill in arterial blood sampling.
How to fill a radial artery?
Advance the needle into the radial artery until a blood flashback appears, then allow the syringe to fill to the appropriate level. DO NOT pull back the syringe plunger.
How to obtain blood gas sample?
The sample can be obtained either through a catheter placed in an artery, or by using a needle and syringe to puncture an artery. These syringes are pre-heparinized and handled to minimize air exposure that will alter the blood gas values. This chapter describes only the procedure for a radial artery blood draw.
How to prevent leaking blood sample?
Expel air bubbles, cap the syringe and roll the specimen between the hands to gently mix it. Cap the syringe to prevent contact between the arterial blood sample and the air, and to prevent leaking during transport to the laboratory.
How to prevent hematoma bleeding?
Haematomaor excessive bleeding can be prevented by inserting the needle without puncturing the far side of the vessel and by applying pressure immediately after blood is drawn. Due to the higher pressure present in arteries, pressure should be applied for a longer time than when sampling from a vein, and should be supervised more closely, to check for cessation of bleeding.
Why is my blood test inaccurate?
Reasons for an inaccurate blood result include: presence of air in the sample; collection of venous rather than arterial blood; an improper quantity of heparin in the syringe, or improper mixing after blood is drawn;
Which artery is used to obtain a sample?
The radial artery on the wrist is most commonly used to obtain the sample. However, the femoral artery and brachial artery can be used if necessary. If the patient already has a pre-existing arterial line, this can be used to obtain the sample⁴.
Why do nurses draw arterial samples?
The main issue is if the patient does not have a functioning arterial line , a frontline clinician has to draw the arterial sample. Some hospitals allow specially trained nurses or phlebotomists to perform this skill but only after an intense training program. If a provider is not available to perform the arterial stick, treatment could be delayed.
What is an Arterial Blood Gas (ABG)?
An ABG is a blood test commonly performed in the ICU and ER setting; however, ABGs can be drawn on any patient on any floor depending on their diagnosis.
What is an ABG test?
What is an Arterial Blood Gas (ABG)? An ABG is a blood test that measures the acidity, or pH, and the levels of oxygen (O2) and carbon dioxide (CO2) from an artery.² The test is used to check the function of the patient’s lungs and how well they are able to move oxygen into the blood and remove carbon dioxide. ...
What is the blood test for oxygen?
An arterial blood gases (A BG) test is a blood test that measures the acidity, or pH, and the levels of oxygen (O2) and carbon dioxide (CO2) from an artery. The test is used to check the function of the patient’s lungs and how well they are able to move oxygen into the blood and remove carbon dioxide.
What is the normal pH of blood gas?
According to the National Institute of Health, typical normal values are: pH: 7.35-7.45. Partial pressure of oxygen (PaO2): 75 to 100 mmHg. Partial pressure of carbon dioxide (PaCO2): 35-45 mmHg.
Where to draw blood for arterial gas?
Blood can be drawn via an arterial stick from the wrist, groin, or above the elbow. The radial artery on the wrist is most commonly used to obtain the sample. However, the femoral artery and brachial artery can be used if necessary.
What Happens During an Arterial Blood Gas Test?
You are likely to have an arterial blood gas test in a hospital , but your doctor may be able to do it in their office.
What is the procedure called to check for blood flow to your hand?
Before the arterial blood gas test, they may apply pressure to the arteries in your wrist for several seconds. The procedure, called the modified Allen test, checks that blood flow to your hand is normal.
How to lower the chance of bruising after blood draw?
To lower the chance of bruising, you can gently press on the area for a few minutes after the needle comes out.
What is the blood gas test?
An arterial blood gas (ABG) test measures oxygen and carbon dioxide levels in your blood. It also measures your body’s acid-base (pH) level, which is usually in balance when you’re healthy.
What test is used to check for breathing problems?
If you are having a hard time breathing, your doctor may use an arterial blood gas (ABG) test to help figure out what the problem is.
How long does it take for a blood gas test to show results?
Arterial Blood Gas Test Results. Results of your arterial blood gas test usually are available in less than 15 minutes.
What is the Allen test?
The procedure, called the modified Allen test, checks that blood flow to your hand is normal. In order to prepare for an arterial blood gas test, tell your doctor about all medications, supplements, and vitamins you’re taking.
How is arterial blood collected?
Arterial Blood: The arterial blood is collected by the direct puncture of an artery.
Where is venous blood found?
Venous Blood: Venous blood is the blood that has passed through various blood capillaries of various organs except for the lungs, and is found in veins, right chambers of the heart, and pulmonary artery.
What is the color of blood in the venous end?
Figure 2: Formation of Venous Blood. Since blood in the venous end is deoxygenated, the color of the blood is blackish red. This deoxygenated blood moves through the venules to the veins. Ultimately, all deoxygenated blood from the body comes to the right atrium of the heart through superior and inferior vena cava.
What is the difference between a pulmonary vein and an arterial vein?
The main difference between arterial and venous blood is that arterial blood is oxygenated whereas venous blood is deoxygenated. The arterial blood is bright red in color and the venous blood is blackish red in color. However, pulmonary artery and pulmonary vein are two exceptions to this; pulmonary artery carries deoxygenated blood away from the heart while pulmonary vein carries oxygenated blood towards the heart.
What is the blood test for oxygen?
It is used to check the efficiency of the lungs to remove carbon dioxide from the blood as well as to take oxygen into the blood. Oxygenated and deoxygenated blood drops are shown in figure 1.
What is the blood that flows from the heart to the metabolizing tissues throughout the body?
The arterial blood is rich in oxygen and other nutrients such as glucose, amino acids, and vitamins. It flows from the heart to the metabolizing tissues throughout the body to supply oxygen and nutrients to the cells.
Which blood type is rich in oxygen and nutrients such as glucose, amino acids, and vitamins?
Arterial Blood : The arterial blood is rich in oxygen and nutrients such as glucose, amino acids, and vitamins. Venous Blood: The venous blood is rich in HCO 3 and metabolic wastes such as urea.
Why do we collect arterial blood?
The most common reason for collection of arterial blood is the evaluation of arterial blood gases. Arterial blood may be obtained directly from the artery (most commonly, the radial artery) by personnel who are trained to perform this procedure and are knowledgeable about the complications that could occur as a result of this procedure. Arterial blood may also be obtained from a vascular access device (VAD) inserted in an artery such as a femoral arterial line or catheter.
Where is capillary blood obtained?
Capillary Blood. Capillary blood is obtained from capillary beds that consist of the smallest veins (venules) and arteries (arterioles) of the circulatory system. The venules and arterioles join together in capillary beds forming a mixture of venous and arterial blood.
What is the blood that flows from the capillary veins to the right side of the heart?
Venous Blood. Venous blood is deoxygenated blood that flows from tiny capillary blood vessels within the tissues into progressively larger veins to the right side of the heart. Venous blood is the specimen of choice for most routine laboratory tests.
What is a VAD in blood?
At times, venous blood may be obtained using a vascular access device (VAD) such as a central venous pressure line or Hickmann Catheter or an IV start. Most laboratory reference ranges for blood analytes are based on venous blood.
What is the specimen of choice for a dermal puncture?
The specimen from a dermal puncture will therefore be a mixture of arterial and venous blood along with interstitial and intracellular fluids. Capillary blood is often the specimen of choice for infants, very young children, elderly patients with fragile veins, and severely burned patients.
