what is serotyping used for

What is the role of serotyping in clinical diagnosis?

Serotyping has been used to categorize most pathogenic members of Pasteurellaceae of human or animal importance. In particular, serotyping of isolates from vaccine breaks is of primary importance to ensure that vaccines used are covering outbreak isolates in the region.

How is serotyping done?

The traditional method to determine a Salmonella serotype is a phenotypic method, based on the WKL scheme (Grimont and Weill, 2007). The serotype is determined by agglutination of the bacteria with specific antisera to identify variants of somatic (O) and flagella (H) antigens.

Why is serotyping particularly important in Salmonella infections?

Why is serotyping particularly important in Salmonella infections and Typhoid fever? Salmonella enterica serotype Enteritidis causes mild self-limiting disease but Salmonella enterica serotype Typhimurium requires treatment. The serotype determines the disease caused and the treatment needed (if any).

What is the serotype of a virus?

A serotype is defined as a variation within a microbial species, distinguished by the humoral immune response. The serotype classification of bacteria or viruses is based on their surface antigens and was established before the availability of other techniques, such as genome sequencing or mass spectrometry.

What is serotyping E. coli?

coli O- and H-types, molecular serotyping generally refers to genetic-based assays targeting O-group-specific genes found within the E. coli O-antigen gene clusters and the H-antigen genes that encode for the different flagellar types.

What is serotype in vaccine?

A serotype is defined as one which either exhibits no cross-reaction with others, or shows a homologous/heterologous titer ratio greater than 16 (in both directions). From: Virus Taxonomy, 2005.

What are the 5 symptoms of a salmonella infection?

What are the symptoms of salmonella infections?Diarrhea.Fever.Abdominal cramps.Chills.Headache.Nausea or vomiting.

Why do antibiotics not treat Salmonella?

Antibiotics are not helpful in most cases of salmonella infection. In fact, antibiotics may extend the period in which you carry the bacteria and can infect others. They can also increase your risk of getting infected again (relapse).

How is Salmonella bacteremia treated?

Salmonella bacteremia is generally treated with a single bactericidal drug for 10-14 days. Given the resistance trends, life-threatening infections should be treated with both a third-generation cephalosporin and a fluoroquinolone until the susceptibilities of antimicrobial agents are known.

Are serotypes and strains the same thing?

Serotypes may be viewed as a further categorization of strains. This is because of the fact that strains of different microorganisms can also have varying antigens and thus a need to categorize this further based on these molecules.

Is there a difference between strain and serotype?

That strain has demonstrably different properties from the human strain. There are other terms to describe viruses but they are more confusing than contentious, and they are not used universally. The term serotype is used to describe viruses of the same species that are antigenically different.

What is difference between different serotypes of a virus?

Posted April 24, 2020. While subtype is simply a broad term for any subclassification under a group, a serotype is a grouping of viruses or other microorganisms based on the type of surface antigens present. Serotyping can be done using a variety of assays, including ELISA and RT-PCR.

What are examples of serovar?

A serotype or serovar is a distinct variation within a species of bacteria or virus or among immune cells of different individuals. These microorganisms, viruses, or cells are classified together based on their surface antigens, allowing the epidemiologic classification of organisms to the subspecies level.

Are serotypes and strains the same thing?

Serotypes may be viewed as a further categorization of strains. This is because of the fact that strains of different microorganisms can also have varying antigens and thus a need to categorize this further based on these molecules.

What is biotype in microbiology?

A biotype is a strain distinguished from other microorganisms of the same species by its physiological properties. Biotype can also be taken to mean a group of organisms with the same genotype.

What are the 4 serotypes of dengue?

Dengue is caused by a virus of the Flaviviridae family and there are four distinct, but closely related, serotypes of the virus that cause dengue (DENV-1, DENV-2, DENV-3 and DENV-4). Recovery from infection is believed to provide lifelong immunity against that serotype.