Why is Strawberry Used for DNA Extraction?
- Strawberries are easy to access in most places in the world.
- They are easy for kids to pulverize with their hands.
- Strawberries contain enzymes called pectinases and cellulases that help to break down cell walls, this helps release that precious DNA.
- A strawberry is octoploid. That means they have eight copies of each type of DNA chromosome. ...
What is the purpose of extracting DNA from Strawberry?
What is the purpose of extracting DNA from strawberries? Ripe strawberries are an excellent source for extracting DNA because they are easy to pulverize and contain enzymes called pectinases and cellulases that help to break down cell walls. And most important, strawberries have eight copies of each chromosome (they are octoploid), so there is ...
Why to use a strawberry for DNA extraction?
companies. For DNA extraction, detergent is used to lyse the cell so that DNA is released into the solution. Then alcohol is added to cause the DNA to precipitate out. Strawberries are chosen for this particular experiment because strawberry cell has eight copies of the genome, and it would be easier to extract the DNA since it is
How to extract the DNA from a strawberry?
- Chill the rubbing alcohol in the freezer. (You’ll need it later.)
- Mix one half teaspoon of salt, one third cup of water and one tablespoon of dishwashing liquid in a glass or small bowl. Set the mixture aside. ...
- Completely line the funnel with cheesecloth. ...
- Remove and discard the green tops from the strawberries.
How do you extract DNA from strawberries?
- Put the strawberries into a resealable plastic sandwich bag and push out all of the extra air. ...
- With your fingers, squeeze and smash the strawberries for two minutes. ...
- Add three tablespoons of the extraction liquid you prepared to the strawberries in the bag. ...
- Squeeze the strawberry mixture with your fingers for one minute. ...
What was the purpose of mashing up the strawberry DNA extraction lab?
What's the purpose of mashing up the berries? DNA is not soluble in alcohol. Adding alcohol to the mashed berries mixture isolates the DNA from the strawberry mixture, hence the reason why the white stringy DNA develops at the top of the mixture.
What is the purpose of fruit DNA extraction?
We will extract DNA from fruit to investigate how it looks and feels. This procedure is similar to what scientists have to do before they can use the information contained in this DNA. This information can be used to improve crops so that they are more resistant to disease, insect invasion or changes in climate.
What are three reasons why we would need to extract DNA?
DNA is extracted from human cells for a variety of reasons. With a pure sample of DNA you can test a newborn for a genetic disease, analyze forensic evidence, or study a gene involved in cancer.
What is the conclusion of DNA extraction experiment?
DNA Extraction Conclusion. REE: The results from our experiment have shown that we were not able to spool DNA from eukaryotic cells. When we added the EtBr to the sample, it did not glow under UV light, unlike the positive control of Salmon Sperm DNA.
What fruits can you extract DNA from and why?
Experiment to purify DNA from fruit Bananas, kiwis and strawberries all work well. (Remove the skin of the bananas and kiwi, we just want the insides!) Step 2: In a separate bowl, mix the washing up liquid, salt and tap water. Stir gently trying to avoid making too many bubbles in the mixture.
What is the purpose of blending in DNA extraction?
To extract DNA, you must remove it from the cells of the DNA source such as the split peas. Blending the peas with some salt and water breaks the cells apart. However, the DNA is still safely contained in the cell because of the cell and nuclear membranes.
Why do we need to mash the fruit in isolating DNA?
Explain that crushing the bananas separates its cells and exposes them to the soap and salt. The soap helps break down cell membranes and release DNA. The salt helps bring the DNA together, and the cold alcohol helps the DNA precipitate and come out of solution so it can be collected.
What can you do with extracted DNA?
Once extracted, DNA can be used for molecular analyses including PCR, electrophoresis, sequencing, fingerprinting and cloning.
Background
DNA is present in every cell of plants and animals. The structure of DNA is a double helix comprised of 4 nucleotides. Each nucleotide has the following:
Preparation
Place one strawberry per student in a plastic baggie and remove most of the air. Mash the strawberries with your hands. Carefully strain the mashed strawberry over a bowl or beaker and retain the liquid portion.
Objective
Determine whether is easier to extract DNA from under-ripe, ripe, or over-ripe strawberries.
Introduction
DNA, or Deoxyribonucleic Acid, is the molecule of life. DNA exists in every single organism, from the smallest bacteria to the largest mammal, and is the only known molecule that has the ability to replicate itself.
