What is thioglycollate medium used for?
Fluid Thioglycollate Medium. Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria. Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar.
What is the composition of thioglycollate?
Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria. Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar. The sodium thioglycollate, thioglycollic acid, and L-cystine reduce the oxygen to ...
What is thioglycollate broth used for?
Thioglycollate broth (Fluid Thioglycollate Medium) is a medium designed to test the aerotolerance of bacteria.
What is the function of sodium thioglycollate?
The sodium thioglycollate, thioglycollic acid, and L-cystine reduce the oxygen to water. Methylene blue is an indicator that is colorless in an anaerobic environment and greenish-blue in the presence of oxygen.
How does sodium thioglycollate reduce oxygen?
Sodium chloride: It maintains osmotic equilibrium. Sodium Thioglycollate: Sodium Thioglycollate is a reducing agent which maintains a low oxygen tension by removing molecular oxygen from the environment i.e., it creates anaerobic conditions when it reduces molecular oxygen to water.
How is oxygen removed from the fluid Thioglycollate medium?
Fluid Thioglycollate: Oxygen Requirements Mechanism/Reactions: This is a nutritive medium with a reducing agent (sodium thioglycollate), which, due to a chemical reaction, removes oxygen from the broth. A chemical indicator is included in the broth- resazurin. The pinkish color indicates the presence of oxygen.
Where is there more oxygen in the fluid Thioglycollate medium?
0:569:34Basics of Fluid Thioglycollate Media (FTM) | With Examples - YouTubeYouTubeStart of suggested clipEnd of suggested clipAnd towards the top of the media in all of these the top has high oxygen levels. The bottom of theMoreAnd towards the top of the media in all of these the top has high oxygen levels. The bottom of the media has low oxygen levels and so what happens is we basically create an oxygen gradient.
Where is the oxygen concentration the lowest in a tube of fluid Thioglycollate medium?
Microaerophile distribution in a fluid thioglycollate medium? Towards the top, but not at the top, so they can have minimal oxygen exposure.
What is the purpose of fluid thioglycollate medium?
Fluid Thioglycollate Medium is a general-purpose medium for the cultivation of anaerobes, microaerophiles and aerobes, and is recommended as one of the media for the sterility testing of biologics.
What does thioglycollate do when it is used in media?
Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. It acts as an enrichment broth which is most frequently used in diagnostic bacteriology....Composition of Thioglycollate Broth.IngredientsGms/literSodium thioglycollate0.55 more rows•Jan 7, 2022
What is the indicator in fluid thioglycollate and what is its purpose?
In Fluid Thioglycollate Medium with indicator, resazurin is an oxidation-reduction indicator, being pink when oxidized (as oxygen is absorbed into the medium) and colorless when reduced.
What is the role of oxygen in aerobic respiration?
Oxygen is the final electron acceptor at the end of the electron transport chain of aerobic respiration. In the absence of oxygen, only a few ATP are produced from glucose. In the presence of oxygen, many more ATP are made.
What are the 3 types of oxygen requirements in bacteria?
SummaryClassificationCharacteristicsFacultative anaerobesWill respire aerobically until oxygen is depleted and then ferment or respire anaerobicallyObligate anaerobesLack superoxide dismutase Generally lack catalase Are fermenters Can not use oxygen as terminal electron acceptor2 more rows•May 5, 2022
How does oxygen affect the growth of bacteria?
Bacteria that require oxygen to grow are called obligate aerobic bacteria. In most cases, these bacteria require oxygen to grow because their methods of energy production and respiration depend on the transfer of electrons to oxygen, which is the final electron acceptor in the electron transport reaction.
Why is it important to get rid of oxygen in the bacterial environment of anaerobes name specific examples of what needs to be removed?
Oxygen Toxicity Obligate anaerobes, which live only in the absence of oxygen, do not possess the defenses that make aerobic life possible and therefore cannot survive in air. The excited singlet oxygen molecule is very reactive. Therefore, superoxide must be removed for the cells to survive in the presence of oxygen.
Can aerobic bacteria grow in the absence of o2?
There are two main types of aerobic bacteria: 1. The obligate aerobes that compulsorily require oxygen for deriving energy, growth, reproduction, and cellular respiration. These organisms do not survive in the absence of oxygen or flooding.
What is the role of resazurin in fluid Thioglycollate Medium?
In Fluid Thioglycollate Medium with indicator, resazurin is an oxidation-reduction indicator, being pink when oxidized (as oxygen is absorbed into the medium) and colorless when reduced.
What does thioglycollate do in an anaerobic culture medium?
Sodium thioglycollate is a reducing agent which maintains a low oxygen tension in anaerobic media. A small amount of agar is added to impede diffusion of oxygen.
What is the role of resazurin in fluid Thioglycollate Medium quizlet?
What is the purpose of Resazurin in thioglycollate media? It is the oxygen indicator.
What is the purpose of adding resazurin indicator in fluid thioglycollate broth?
Resazurin is an oxidation-reduction indicator that turns pink when increased oxidation has occurred, it is colorless when reduced. Agar: The addition of a small amount of agar in Thioglycollate medium aids in the initiation and growth of small inocula and anaerobes by impeding the diffusion of oxygen into the medium.
What is fluid thioglycolate medium?
Fluid Thioglycolate Medium (FTM) is a universal complex medium. Thioglycolate and L-Cystine in the medium reduce the redox potential to create an anaerobic atmosphere. Additionally, they inactivate mercury and other heavy metal compounds. Agar further reduces the rapid diffusion of oxygen through the medium but may lead to slight turbidity in ...
Does agar reduce oxygen?
Agar further reduces the rapid diffusion of oxygen through the medium but may lead to slight turbidity in larger volumes (filled bottles). Resazurin indicates the reduction potential of the medium, and a color change to pink indicates an increased concentration of oxygen.
What is thioglycollate medium used for?
Thioglycollate Medium is recommended for sterility testing of biologicals and for the cultivation of anaerobes, aerobes, and microaerophiles from pharmaceutical as well as clinical samples.
What is Brewer's Fluid Thioglycollate Medium?
Brewer formulated Fluid Thioglycollate Medium for rapid cultivation of aerobes as well as anaerobes including microaerophiles by including a reducing agent and small amount of agar.
What is the best medium for sterility testing?
Thioglycollate broth is the recommended medium for sterility testing of antibiotics, biologicals, and foods and for determining the phenol coefficient and sporicidal effect of disinfectants.
Why do microaerophiles need oxygen?
Microaerophilic organisms: Growth at the upper part of the thioglycollate broth since they need oxygen because they cannot ferment or respire anaerobically. However, high concentrations of oxygen may be fatal.
What is aerotolerant growth?
Aerotolerant organisms: Uniform growth in the whole broth since they do not require oxygen as they metabolize energy anaerobically. Unlike obligate anaerobes, though, they are not deadly by oxygen.
Why can't you reheat thyoglycolate broth?
Thioglycolate broth should not be re-heated more than once because toxic oxygen radicles are formed on reheating.
What conditions must be maintained for the optimal recovery of microorganisms?
Proper anaerobic conditions must be maintained for the optimal recovery of microorganisms.
General description
Fluid Thioglycolate Medium is a universal complex medium with thioglycollate and L-Cystine to reduce the redox potential of the culture medium and create an anaerobic atmosphere. They also inactivate mercury and other heavy metal compounds.
Application
Fluid Thioglycolate Medium is suitable for the isolation and cultivation of fastidious anaerobic as well as for aerobic microorganisms. It is used for sterility control of pharmaceutical products.
Features and Benefits
Our ready to use media provide the highest level of quality and testing confidence. They have been formulated and tested to meet the pharmacopoeia requirements. Sterility testing media and rinse solutions are manufactured in an ISO 9001, environmentally controlled production center.